Evaluation of STR profiles of single telogen hairs using probabilistic methods. Issue 1 (December 2019)

• Record Type: Journal Article
• Authors: Heß, Sarah Aurora; Biermann, Jan-Philip; Grabmüller, Melanie; Madea, Burkhard; Thiele, Ralf; Jäger, Richard
• Is Part Of: Forensic science international. Volume 7:Issue 1(2019)
• Page Start: 454
• Page End: 456
• Publication Date: 2019-12
• Subjects: Telogen hair -- Degraded DNA -- Short tandem repeat (STR) -- Forensic genomics -- DNA typing -- Next Generation Sequencing (NGS) -- Probabilistic methods; Forensic genetics -- Periodicals; Forensic Genetics -- Periodicals; Electronic journals; 614.1
• DOI: 10.1016/j.fsigss.2019.10.049
• Languages: English
• ISSNs: 1875-1768

Abstract

Abstract: Full STR profiles can rarely be obtained from single telogen hairs due to the minute amount and compromised integrity of their DNA. We developed a next generation sequencing (NGS)-based STR assay, called maSTR (mini-amplicon STR) assay that is particularly suited for degraded DNA. The maSTR assay yielded higher allele recoveries than capillary electrophoresis (CE)-based STR analysis from DNA from single telogen hairs, however displayed a higher number of allelic drop-ins. To calculate likelihood ratios for assignment to correct donors, profiles composed by consensus or composite interpretation were subjected to fully or semicontinuous probabilistic methods using the GenoProof Mixture 3 software. It was possible to assign profiles from single telogen hairs to their correct donor profiles if DNA was of sufficient quality.