Comprehensive expression analysis of the beta integrin from Penaeus monodon indicating its participation in innate immunity and ammonia nitrogen stress response. Issue 98 (March 2020)

• Record Type: Journal Article
• Authors: Shi, Mengke; Jiang, Shigui; Li, Yundong; Yang, Qibin; Jiang, Song; Yang, Lishi; Huang, Jianhua; Zhou, Falin
• Is Part Of: Fish & shellfish immunology. Issue 98(2020)
• Page Start: 887
• Page End: 898
• Publication Date: 2020-03
• Subjects: Penaeus monodon -- Beta integrin subunit -- dsRNA -- Innate immunity -- Ammonia nitrogen stress -- Apoptosis; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.11.049
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: The aim of the present study was to investigate the function of the beta integrin (PmItgb) in Penaeus monodon . The 3011 bp cDNA sequence of PmItgb was cloned from P. monodon using rapid amplification of cDNA ends (RACE) PCR. Phylogenetic tree analyses indicated that the amino acid sequence of PmItgb should be merged into Fenneropenaeus chinensis (93%). Quantitative real-time PCR (q RT-PCR) revealed that PmItgb mRNA was highly expressed in the hemocytes. In addition, with regard to developmental stages, PmItgb showed significantly higher expression in oosperm, nauplius IV, zoea I and III, and post larval stages than that in other development stages. PmItgb expression in the shrimp epidermis was higher in the postmolt (B) stage, and lower in other molting stages. We also found that Vibrio harveyi and V. anguillarum challenge enhanced PmItgb expression in the hepatopancreas and gills. When PmItgb was inhibited, innate immunity-related genes such as ALF, crustin 1, crustin 7, penaeidin 3, and penaeidin 5 were significantly down-regulated. Furthermore, we demonstrated that PmItgb knock-down by specific dsRNA reduced bacterial clearance. In high ammonia nitrogen concentrations, PmItgb was significantly up-regulated in the hepatopancreas and gills. After PmItgb was silenced, the rate of mortality owing to high ammonia nitrogen concentrations decreased; the expression of related anti-apoptotic genes was up-regulated, and that of the apoptotic genes was slightly down-regulated. These results suggested that PmItgb may be involved in shrimp innate immunity and mediate apoptosis of hepatopancreatic cells induced by high ammonia nitrogen environments. Highlights: The 3011 bp cDNA sequence of the beta integrin (PmItgb) was cloned from Penaeus monodon . The transcripts of PmItgb had a certain pattern in tissues during the whole growth and development stages. PmItgb mRNA was up-regulated after injected by gram-negative bacteria, bacterial clearance was significantly reduced by specific dsRNA knockdown PmItgb. The mortality of shrimp under high ammonia nitrogen environmental stress was delayed following PmItgb knockdown.