Evaluating the amplification efficiency of the MALBAC® single-cell DNA Kit for trace DNA. Issue 1 (December 2019)

• Record Type: Journal Article
• Authors: Xu, Qiannan; Zhang, Jiayi; Li, Li; Li, Chengtao; Liu, Xiling
• Is Part Of: Forensic science international. Volume 7:Issue 1(2019)
• Page Start: 351
• Page End: 352
• Publication Date: 2019-12
• Subjects: Whole genome amplification -- Trace DNA; Forensic genetics -- Periodicals; Forensic Genetics -- Periodicals; Electronic journals; 614.1
• DOI: 10.1016/j.fsigss.2019.10.008
• Languages: English
• ISSNs: 1875-1768

Abstract

Abstract: DNA typing using trace DNA poses a significant challenge for forensic science. To test whether whole genome amplification (WGA) technology could resolve this problem, we first evaluated the efficiency of WGA technology for DNA using the MALBAC® Single-cell DNA Kit to amplify trace DNA and the Goldeneye™ DNA ID System to genotype short tandem repeats (STRs). The amplified DNA yield and the STR-typing success rate were then assessed. The DNA yield was found to increase more than 200-fold following WGA. Moreover, the STR-typing success rate using the amplified DNA with a template < 6.25 pg was higher than that without amplification. This study suggests the potential usability of whole genome amplification technology in forensic trace DNA analysis.