Application of anaerobic dynamic membrane bioreactor (AnDMBR) for the successful enrichment of Anammox bacteria using mixed anaerobic and aerobic seed sludge. (October 2018)

• Record Type: Journal Article
• Authors: Saleem, Mubbshir; Lavagnolo, Maria Cristina; Concheri, Giuseppe; Stevanato, Piergiorgio; Squartini, Andrea; Spagni, Alessandro
• Is Part Of: Bioresource technology. Volume 266(2018)
• Page Start: 532
• Page End: 540
• Publication Date: 2018-10
• Subjects: Dynamic membrane -- Anammox -- Mesh filtration -- Denitrification -- PCR sequence analysis; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2018.06.100
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Successful enrichment of Anammox bacteria was achieved by using DM bioreactor. Solid retention and TN removal of DM was comparable to conventional membrane. DM fouling increased with the progressive enrichment of Anammox bacteria. PCR Sequence analysis confirmed the progressive enrichment of Anammox bacteria. Abstract: This study investigated a novel bioreactor configuration coupled with a side-stream dynamic membrane (DM) for Anammox enrichment as an alternative for conventional membrane. Bioreactor was fed with synthetic feed and seeded with a mix of anaerobic and aerobic sludge. In situ mechanical cleaning was employed for DM cleaning. DM development and performance was analysed over two polyamide-nylon meshes (200 and 52 µm). Solid-liquid separation of 52 µm mesh outperformed 200 µm with an average effluent turbidity of 2.4 ± 0.1 NTU. The system was operated at a maximum nitrogen loading rate of 696 mg-N L −1 d −1 and achieved a maximum nitrogen removal rate of 611.6 mg-N L −1 d −1 . At steady state, the average ammonium, nitrite and total nitrogen removal efficiencies were 87 ± 0.6%, 98.5 ± 0.15% and 87.5 ± 0.56% respectively. Digital realtime PCR Sequence analysis showed that Planctomycetales belonging to ascertained Anammox-specific genera progressively increased their presence in the reactor consistently with its nitrogen removal performance.