Age-related changes of CD4+ T cell migration and cytokine expression in germ-free and SPF mice periodontium. (March 2018)

• Record Type: Journal Article
• Authors: Irie, Koichiro; Tomofuji, Takaaki; Ekuni, Daisuke; Fukuhara, Daiki; Uchida, Yoko; Kataoka, Kota; Kobayashi, Shuichiro; Kikuchi, Takeshi; Mitani, Akio; Shimazaki, Yoshihiro; Morita, Manabu
• Is Part Of: Archives of oral biology. Volume 87(2018)
• Page Start: 72
• Page End: 78
• Publication Date: 2018-03
• Subjects: CCL5 C-C chemokine ligand 5 -- IL interleukin -- RANKL receptor activator of nuclear factor-kappa B ligand -- GF germ free -- SPF specific pathogen free -- CT computed tomography -- MRS de Man Rogosa and sharpe -- BV bone volume -- TV total volume -- Tb.Th trabecular thickness -- Tb.N trabecular number -- Tb.Sp trabecular separation -- CEJ cement-enamel junction -- ABC alveolar bone crest -- M molar -- PCR polymerase-chain reaction -- AMV avian myeloblastosis virus -- GAPDH glyceraldehyde-3-phosphate dehydrogenase -- TRAP tartrate-resistant acid phosphatase; Host response -- Inflammation and innate immunity -- Pathogenesis of periodontal disease -- T-cell biology; Mouth -- Periodicals; Mouth -- Diseases -- Periodicals; Dentistry -- Periodicals; Electronic journals; 617.6005
• DOI: 10.1016/j.archoralbio.2017.12.007
• Languages: English
• ISSNs: 0003-9969

Abstract

Highlights: SPF mice, but not GF mice, showed an age-related increase in alveolar bone loss. The migrated CD4 + T cell were co-expressed with IL-17A and RANKL in SPF mice. GF mice did not show age-related changes in CD4 + T cell migration and cytokine, expression. Abstract: Objective: Increasing age is a potential risk factor for periodontal tissue breakdown, which may be affected by commensal flora. The aim of this study evaluated age-related changes in CD4 + T cells, C-C chemokine ligand 5 (CCL5), interleukin (IL)-17A, and receptor activator of nuclear factor-kappa B ligand (RANKL) expression using germ-free (GF) and conventionally reared (SPF) mice. Design: GF and SPF mice at 8 (n = 6/group) and 22 weeks old (n = 6/group) were used. Immunohistochemical analyses were performed to determine the effects of aging on protein expression in periodontal tissues. Age-related changes in alveolar bone were quantified using micro-CT analysis. Results: SPF mice, but not GF mice, showed an age-related increase in alveolar bone loss ( P < 0.01). SPF mice at 22 weeks of age increased expression of CD4 + T cells, CCL5, IL-17A, and RANKL compared to those at 8 weeks of age in connective tissue and alveolar bone surface ( P < 0.01). Furthermore, there was increased CD4 + T cells, which were co-expressed with IL-17A and RANKL in SPF mice at 22 weeks of age. On the other hand, the GF mice did not show any significant differences in CD4 + T cells, CCL5, IL-17A and RANKL expression between the two age groups. Conclusions: SPF mice induced an age-related increase in CD4 + T cells co- expressed with IL-17A and RANKL, with occurring alveolar bone loss. In contrast, GF mice did not show age-related changes in CD4 + T cell migration and cytokine expression.