Differential angiogenic activities of naringin and naringenin in zebrafish in vivo and human umbilical vein endothelial cells in vitro. (October 2018)

• Record Type: Journal Article
• Authors: Chen, Linmin; Yang, Binrui; Tang, Benqin; Gong, Guiyi; Kam, Hiotong; Gao, Cheng; Chen, Yan; Wang, Ruibing; Lee, Simon Ming Yuen
• Is Part Of: Journal of functional foods. Volume 49(2018)
• Page Start: 369
• Page End: 377
• Publication Date: 2018-10
• Subjects: CVD cardiovascular diseases -- DA dorsal aorta -- ECGS endothelial cell growth supplement -- EGFP enhanced green fluorescent protein -- F-12K Kaighn's modification of Han's F12 medium -- hpf hours post fertilization -- hpt hours post treatment -- HUVEC human umbilical vein endothelial cells -- IHD Ischaemic heart diseases -- ISV intersegmental vessels -- PCV posterior cardinal vein -- SIV subintestinal vessels -- VEGF vascular endothelial growth factor -- VEGFR VEGF Receptor -- VRI VEGFR tyrosine kinase inhibitor II -- WT Wild type; Angiogenesis -- Naringin -- Zebrafish -- HUVEC; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2018.08.010
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Naringin, commonly found in the pericarp of citrus fruits, promoted HUVEC migration. Naringin exhibited restorative effects on chemically-induced blood vessel loss in zebrafish. Naringin has the potential to be developed as a therapeutic agent for diseases associated with insufficient angiogenesis. Abstract: Naringin, a flavanone glycoside, and naringenin, the aglycone of naringin, are commonly found in the pericarp of citrus fruits and have also been considered as potential bioactive flavanones. In the present study, treatment with naringenin showed an inhibitory effect on SIV formation in zebrafish embryos, which demonstrates its potential anti-angiogenic activity. In a chemically-induced blood vessel loss model in zebrafish, we found that naringin exhibited remarkably pro-angiogenesis activity on the restoration of blood vessel loss, and significantly reversed VRI-induced down-regulation of flt1 mRNA expression. In an in vitro study of cultured human umbilical vein endothelial cells (HUVEC), our results showed that naringin specifically promoted HUVEC migration but not proliferation. Taken together, these results suggest that naringin and naringenin have differential angiogenesis activities where naringin, as a novel angiogenic agent, has the potential to be developed as a therapeutic agent for diseases associated with insufficient angiogenesis, such as ischemia heart disease.