Selection of potential bacterial strains to develop bacterial consortia for the remediation of e-waste and its in situ implications. (September 2018)

• Record Type: Journal Article
• Authors: Debbarma, Prasenjit; Zaidi, M.G.H.; Kumar, Saurabh; Raghuwanshi, Shikha; Yadav, Amit; Shouche, Yogesh; Goel, Reeta
• Is Part Of: Waste management. Volume 79(2018)
• Page Start: 526
• Page End: 536
• Publication Date: 2018-09
• Subjects: E-waste -- Bioformulation -- Biodegradation -- FTIR -- TG-DTG-DTA -- SEM; Hazardous wastes -- Periodicals; Refuse and refuse disposal -- Periodicals; 363.728
• DOI: 10.1016/j.wasman.2018.08.026
• Languages: English
• ISSNs: 0956-053X

Abstract

Graphical abstract: Highlights: Strains PE2, PE5, PE8, PE10 and PE15 screened as e-waste utilizing bacteria. FTIR spectra validated that the C-chain in e-waste was tremendously affected. Thermal analysis showed bacteria treated e-waste decomposed at lower temperature. SEM micrographs revealed the surface of the treated e-waste was disintegrated. Bacterial consortia C-1 and C-3 were found effective under in situ conditions. Abstract: The management of electronic waste (e-waste) becomes a global issue in this digital era. Existing conventional practices are harmful for dealing with the e-waste, therefore, indigenous soil bacteria were explored for e-waste treatment through enrichment culture approach followed by screening, identification and their bioformulation used for in situ investigation. Soil bacteria were enriched in the presence of e-waste after 30 days of incubation under standard laboratory conditions. This finding was established through λmax values that were higher in the case of soil enrichment than the control. The biodegradation of e-waste by the selected strains during enrichment and in situ experiment was confirmed by FTIR, TG-DTG-DTA and SEM analysis. The FTIR spectra evidently support that microbial communities present in the enriched soil has affected the C-chain and used as carbon source for their growth. This chemical structural degradation of e-waste was further substantiated by thermal and SEM analysis. Thermograms experimentally show that the decomposition of the treated samples achieved comparatively at very low temperature than the control sample, while SEM micrographs revealed the surface morphology with distinct disintegrations. These result authenticated the biodegradation process carried out by the soil bacteria. Furthermore, bacterial community analysis confirmed that the used strains were persisting in the experimental pits throughout the trial period. Thus, this study besides providing direct and standardized protocol for screening and selection of efficient e-waste utilizing bacteria is also demonstrating potential consortia which are ready to be used.