Solvents production from cassava by co-culture of Clostridium acetobutylicum and Saccharomyces cerevisiae. Issue 1 (February 2018)

• Record Type: Journal Article
• Authors: Qi, Gaoxiang; Xiong, Lian; Luo, Mutan; Huang, Qianlin; Huang, Chao; Li, Hailong; Chen, Xuefang; Chen, Xinde
• Is Part Of: Journal of environmental chemical engineering. Volume 6:Issue 1(2018)
• Page Start: 128
• Page End: 133
• Publication Date: 2018-02
• Subjects: Acetone-butanol-ethanol fermentation -- Cassava -- Clostridium acetobutylicum -- Co-culture -- Saccharomyces cerevisiae; Chemical engineering -- Environmental aspects -- Periodicals; Environmental engineering -- Periodicals; Chemical engineering -- Environmental aspects; Environmental engineering; Periodicals; 660.0286
• DOI: 10.1016/j.jece.2017.11.067
• Languages: English
• ISSNs: 2213-2929

Abstract

Highlights: Cassava was used for solvents fermentation without amylolytic enzymes addition. Co-culture of Clostridium acetobutylicum and Saccharomyces cerevisiae was conducted. Effect of different inoculation methods on solvents titer and yield was evaluated. 42.56 g/L ABE was achieved from 150 g/L cassava by co-culture process. Abstract: Co-culture of Clostridium acetobutylicum and Saccharomyces cerevisiae was used to enhance solvents (acetone-butanol-ethanol, ABE) production from cassava without amylolytic enzymes addition. Under the simultaneous inoculation condition of C. acetobutylicum and S. cerevisiae with the inoculation level of 5% (v/v) for both strains, 21.74 g/L solvents was produced from 75 g/L cassava with the yield of 0.36 g solvents/g glucose. The solvents titer and yield obtained in the co-culture process increased by 14.3% and 9.1%, respectively, compared to those achieved in ABE fermentation. For further improvements in total solvents titer and yield from cassava, ABE fermentation and co-culture process from higher cassava concentration media were conducted. 42.56 g/L total solvents was achieved from 150 g/L cassava by co-culture with the yield of 0.46 g solvents/g glucose. The solvents titer and yield obtained in the co-culture process increased by 141.0% and 43.8%, respectively, compared to those obtained from ABE fermentation. The results showed great potential for the use of co-culture of C. acetobutylicum and S. cerevisiae for solvents production from starchy substrate.