Vitamin D suppresses macrophage infiltration by down-regulation of TREM-1 in diabetic nephropathy rats. (15th September 2018)

• Record Type: Journal Article
• Authors: Zhao, Yu; Guo, Yinfeng; Jiang, Yuteng; Zhu, Xiaodong; Zhang, Xiaoliang
• Is Part Of: Molecular and cellular endocrinology. Volume 473(2018)
• Page Start: 44
• Page End: 52
• Publication Date: 2018-09-15
• Subjects: Active vitamin D -- Macrophage -- Adhesion -- Migration -- TREM-1 -- DN; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2018.01.001
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: This study intends to investigate the effect of active vitamin D (VD) on the expression of triggering receptor expressed on myeloid cells-1 (TREM-1) in the renal tissues of diabetic nephropathy (DN) rats and to explore the impact of TREM-1 on macrophage adhesion and migration. We find that the expressions of TREM-1 and CD68 protein are higher in DN rats compared with rats in the normal control group and that these changes are decreased in the DN + VD group. In vitro, the capacity for macrophage adhesion and migration and the expression of TREM-1 are increased under high-glucose conditions, but VD inhibits this progress. TREM-1 siRNA decreases high-glucose-induced macrophage adhesion and migration, whereas over-expression of TREM-1 inhibits its action. However, VD cannot suppress high glucose-induced TREM-1 expression and macrophage adhesion and migration when TREM-1 is over-expressed. These results demonstrate that VD can suppress macrophage adhesion and migration by reducing the expression of TREM-1. Highlights: Active vitamin D ameliorates renal damage and reduces the expression of TREM-1 and macrophage infiltration in STZ-induced diabetic nephropathy rats. Active vitamin D decreases high glucose-induced TREM-1 expression and macrophage adhesion and migration in vitro. TREM-1 over-expression increases high-glucose-induced macrophage adhesion and migration in vitro. Active vitamin D cannot suppress high glucose-induced TREM-1 expression and macrophage adhesion and migration when TREM-1 is over-expressed.