C-reactive protein/serum amyloid P promotes pro-inflammatory function and induces M1-type polarization of monocytes/macrophages in mudskipper, Boleophthalmus pectinirostris. Issue 94 (November 2019)

• Record Type: Journal Article
• Authors: Cai, Shi-Yu; Nie, Li; Chen, Jiong
• Is Part Of: Fish & shellfish immunology. Issue 94(2019)
• Page Start: 318
• Page End: 326
• Publication Date: 2019-11
• Subjects: CRP/SAP -- Monocyte/macrophage -- Mudskipper -- Phagocytosis -- Polarization -- Sequence analysis; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.09.021
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: C-reactive protein (CRP) and serum amyloid P (SAP) play essential roles in the phagocytic cell-mediated innate immune response of mammals. In-depth studies into CRP and SAP have been completed in mammals; however, such studies, particularly those relating to the functions of CRP and SAP, are rare in fish species. In this study, a homolog of CRP/SAP (BpCRP/SAP) was identified in mudskipper ( Boleophthalmus pectinirostris ), which had the typical characteristics of a fish short pentraxin protein. Phylogenetic tree analysis revealed that BpCRP/SAP was most closely related to mudskipper CRP/SAP-l3. BpCRP/SAP transcripts were detected in all tested tissues, with the highest level observed in the liver; transcripts in the immune tissues and protein expression in the serum were induced in response to Edwardsiella tarda infection. The active recombinant BpCRP/SAP (rBpCRP/SAP) was able to augment the mRNA expression of pro-inflammatory cytokines and attenuate the mRNA expression of anti-inflammatory cytokines in monocytes/macrophages (MO/MΦ). In addition, phagocytosis and bacterial killing of E. tarda by mudskipper MO/MΦ were boosted by rBpCRP/SAP stimulation. rBpCRP/SAP also promoted M1-type MO/MΦ polarization, but inhibited M2-type polarization. In conclusion, the present research describes the pro-inflammatory function of BpCRP/SAP in mudskipper against E. tarda infection. Highlights: We characterized a short-chain PTX (BpCRP/SAP) gene from mudskipper. BpCRP/SAP is a positive APP in mudskipper responding to Edwardsiella tarda infection. rBpCRP/SAP promotes pro-inflammatory function of mudskipper MO/MΦ. rBpCRP/SAP induces M1 type polarization of mudskipper MO/MΦ.