Impedimetric quantification of anti-dengue antibodies using functional carbon nanotube deposits validated with blood plasma assays. (1st June 2018)

• Record Type: Journal Article
• Authors: Palomar, Quentin; Gondran, Chantal; Marks, Robert; Cosnier, Serge; Holzinger, Michael
• Is Part Of: Electrochimica acta. Volume 274(2018)
• Page Start: 84
• Page End: 90
• Publication Date: 2018-06-01
• Subjects: Carbon nanotube deposits -- Electrochemical impedance spectroscopy -- Dengue virus antibody -- Immunosensor; Electrochemistry -- Periodicals; Electrochemistry, Industrial -- Periodicals; 541.37
• DOI: 10.1016/j.electacta.2018.04.099
• Languages: English
• ISSNs: 0013-4686

Abstract

Abstract: A high performance impedimetric immunosensor for the dengue virus antibody detection is presented. The setup profits from the formation of controlled and reproducible carbon nanotube (CNT) deposits on electrodes. Their easy functionalization via electrogeneration of a polypyrrole-NHS (N-hydroxysuccinimido 11-(pyrrol-1-yl) undecanoate) film enables the immobilization of the Dengue Virus 2 NS1 glycoprotein, the receptor unit, on the porous CNT layer via covalent amide coupling to provide the necessary selectivity towards Dengue NS1 antibody. All building steps of this immunosensor and the performance of this system were monitored by impedance spectroscopy and cyclic voltammetry. The resulting impedimetric dengue biosensor was tested in bovine blood plasma in addition to conventional measurements under controlled environment. After optimization, this immunosensor shows a good linearity in a wide concentration range (10 −13 –10 −5 g mL −1 ). Graphical abstract: Image 1 Highlights: A high performing impedimetric immunosensor for dengue virus antibody is presented. Controlled carbon nanotube deposits improved linear range and detection limit. The equivalent circuit allows the determination of various biosensor parameters. The architecture was validated in bovine blood plasma.