Applications of epigenetic methylation in body fluid identification, age determination and phenotyping. Issue 1 (December 2019)

• Record Type: Journal Article
• Authors: McCord, Bruce; Gauthier, Quentin; Alghanim, Hussain; Antunes, Joana; Fernandez Tejero, Nicole; Duncan, George; Balamurugan, Kuppareddi
• Is Part Of: Forensic science international. Volume 7:Issue 1(2019)
• Page Start: 485
• Page End: 487
• Publication Date: 2019-12
• Subjects: Epigenetic -- Serology -- Smoking status -- Pyrosequencing -- High resolution melt; Forensic genetics -- Periodicals; Forensic Genetics -- Periodicals; Electronic journals; 614.1
• DOI: 10.1016/j.fsigss.2019.10.061
• Languages: English
• ISSNs: 1875-1768

Abstract

Abstract: In this paper, four different applications are presented in forensic epigenetics using bisulfite modified PCR, HRM, and pyrosequencing. The first application involves the development of a multiplex amplification capable of simultaneous analysis of 4 different body fluid/cell types- blood, saliva, sperm, and vaginal epithelia. We have also developed a method for the detection of sperm high-resolution melt analysis (HRM). Two other applications involve a determination of suspect age and a biomarker for determining smoking status.