Boosting bioethanol production from Eucalyptus wood by whey incorporation. (February 2018)

• Record Type: Journal Article
• Authors: Cunha, Manuel; Romaní, Aloia; Carvalho, Margarida; Domingues, Lucília
• Is Part Of: Bioresource technology. Volume 250(2018)
• Page Start: 256
• Page End: 264
• Publication Date: 2018-02
• Subjects: Bioethanol -- Cheese whey -- Lignocellulose biomass -- β-Galactosidase -- High solid loadings; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2017.11.023
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: A multi-waste valorization approach was proposed for bioethanol production. SSF of residues mixture was optimized with an industrial strain using high solids loading. High ethanol concentration was obtained by incorporating cheese whey in Eucalyptus SSF. S. cerevisiae Ethanol Red® was engineered for β-galactosidase production. Recombinant strain was used for ethanol production by SSF of residues mixture. Abstract: The mixture of Eucalyptus globulus wood (EGW) and cheese whey powder (CWP) was proposed for intensification of simultaneous saccharification and fermentation (SSF) at high temperature and solid loadings using the industrial Saccharomyces cerevisiae Ethanol Red® strain. High ethanol concentration (93 g/L), corresponding to 94% ethanol yield, was obtained at 35 °C from 37% of solid mixture using cellulase and β-galactosidase enzymes (24.2 FPU/g and 20.0 U/g, respectively). The use of CWP mixed with pretreated EGW increased the ethanol concentration in 1.5-fold, in comparison with SSF experiments without CWP for both Ethanol Red® and CEN.PK113-7D strains. Moreover, 1.4-fold higher ethanol concentration was obtained with Ethanol Red®, in comparison with CEN.PK113-7D strain. Ethanol Red® strain was genetically engineered for β-galactosidase production in order to advance towards a fully integrated process. This work shows the feasibility of attaining high ethanol concentrations in second generation bioprocesses by a multi-waste valorization approach.