Contrast-induced nephropathy in an animal model: Evaluation of novel biomarkers in blood and tissue samples. (2019)

• Record Type: Journal Article
• Authors: Mamoulakis, Charalampos; Fragkiadoulaki, Irene; Karkala, Phaedra; Georgiadis, Georgios; Zisis, Ioannis-Erineos; Stivaktakis, Polychronis; Kalogeraki, Alexandra; Tsiaoussis, Ioannis; Burykina, Tatyana; Lazopoulos, George; Tsarouhas, Konstantinos; Kouretas, Dimitrios; Tsatsakis, Aristides
• Is Part Of: Toxicology reports. Volume 6(2019)
• Page Start: 395
• Page End: 400
• Publication Date: 2019
• Subjects: ADMA asymmetric dimethylarginine -- AVMA American Veterinary Medical Association -- AKI acute kidney injury -- ANOVA analysis of variance -- ARRIVE animal research: reporting of in vivo experiments -- BNMN Bi-nucleated cells with micronuclei -- CBPI cytokinesis block proliferation index -- CIN contrast-induced nephropathy -- CKD chronic kidney disease -- CM contrast medium -- ESI electrospray ionization -- GFR glomerular filtration rate -- KIM-1 kidney injury molecule-1 -- LC–MS liquid chromatography mass spectrometry -- MN micronuclei -- NO nitric oxide -- NGAL meutrophil gelatinase–associated lipocalin -- OECD Organisation for Economic Co-operation and Development -- RBF renal blood flow -- ROS reactive oxygen species -- SCR serum creatinine -- SD standard deviation -- SDMA symmetric dimethylarginine -- TPT touch preparation technique; Asymmetric dimethylarginine -- Contrast media -- Kidney -- Models -- Animal -- Iopromide -- Nephropathy -- Nephrotoxicity -- Symmetric dimethylarginine; Toxicology -- Periodicals; Clinical toxicology -- Periodicals; Drug-Related Side Effects and Adverse Reactions; Hazardous Substances; Poisoning; Toxicology; Electronic journals; Periodicals; Periodicals; 571.9505
• DOI: 10.1016/j.toxrep.2019.04.007
• Languages: English
• ISSNs: 2214-7500

Abstract

Graphical abstract: Highlights: Pathophysiology of contrast induced nephropathy is complex and obscure. Iopromide increases sCr, SDMA and ADMA levels. Iopromide increases significantly micronuclei frequency in lymphocytes. Iopromide enhances cell degeneration and apoptosis in renal tissues. Abstract: Identification of novel biomarkers of contrast-induced nephropathy (CIN) that may more accurately detect renal function changes; reflect kidney damage; assist monitoring; and elucidate pathophysiology attract considerable scientific attention nowadays. To evaluate novel biomarkers of nephrotoxicity in blood/tissue samples of a CIN model, 10 New Zealand white rabbits were divided into group 1 (n = 5; iopromide) and group 2 (n = 5; control). Blood was drawn at 0 h (immediately), 24 h and 48 h after contrast medium (CM) administration. Animals were euthanized at 48 h and kidneys were removed. Serum creatinine (sCr)/symmetric-asymmetric dimethylarginine (SDMA-ADMA) levels were measured. CM genotoxic/cytotoxic effect was investigated 48 h post-CM exposure using micronucleus assay in lymphocytes. Cytological examination was conducted using touch preparation technique (TPT). All animals in group 1 developed CIN: mean sCr levels increased by 68.2% within 48 h. Significant SDMA-ADMA level elevation was observed at 0 h and 24 h with insignificant drop at 48 h in group 1, remaining normal in group 2 at all time-points. Significant increase in bi-nucleated cells with micronuclei and micronuclei frequency was detected in group 1. Cytokinesis block proliferation index was reduced insignificantly in group 1. TPT revealed degenerative lesions/inflammation, cell degeneration, abnormal uterine tubular casts and rubella in kidneys of all animals in group 1. Group 2 presented normal cells.