Duration of protection and humoral immunity induced by an adenovirus-vectored subunit vaccine for foot-and-mouth disease (FMD) in Holstein steers. Issue 42 (30th September 2019)

• Record Type: Journal Article
• Authors: Sitt, Tatjana; Kenney, Mary; Barrera, José; Pandya, Mital; Eckstrom, Korin; Warner, Megan; Pacheco, Juan M.; LaRocco, Michael; Palarea-Albaladejo, Javier; Brake, David; Rieder, Elizabeth; Arzt, Jonathan; Barlow, John W.; Golde, William T.
• Is Part Of: Vaccine. Volume 37:Issue 42(2019)
• Page Start: 6221
• Page End: 6231
• Publication Date: 2019-09-30
• Subjects: Foot-and-mouth disease virus -- Cattle -- Replication-deficient human adenovirus vectored vaccine -- FMDV A24/Cruzeiro/BRA/55 -- Duration of protection -- Humoral immunity; Ad5 human replication deficient adenovirus serotype 5 -- AdtFMD commercial replication-deficient human adenovirus vectored FMD empty capsid -- Adt.24 commercial replication-deficient human adenovirus vectored FMD expressing the capsid proteins from strain A24 Cruzeiro -- ASCs antibody secreting cells -- BID50 BID50, bovine infectious dose 50% -- DIVA differentiate infected from vaccinated animals -- dpc days post-challenge -- dpv days post-vaccination -- FFB final formulation buffer -- FMD foot-and-mouth disease -- FMDV foot-and-mouth disease virus -- IDL intradermolingual -- IM intramuscular -- mpv months post-vaccination -- MT mean VNT titer in log10 scale -- OIE Office International des Epizooties (now World Organisation for Animal Health) -- PBMC Peripheral blood mononuclear cells -- PIADC Plum Island Animal Disease Center's -- SC subcutaneous -- TD transdermal -- UVM MREC University of Vermont, Paul Miller Research and Educational Center -- VNT virus neutralization titer; Vaccines -- Periodicals; 615.372
• DOI: 10.1016/j.vaccine.2019.08.017
• Languages: English
• ISSNs: 0264-410X

Abstract

Highlights: Vaccination with Adt.24 confers protection against FMD for six months. Partial protection was demonstrated after six months. Virus neutralizing titers do not correlate to protection. Anti-FMDV antibody secreting cells are not detected in peripheral blood after 5 weeks following vaccination with Adt.A24. Abstract: Foot-and-mouth disease (FMD) is a highly contagious viral infection of cloven hooved animals that continues to cause economic disruption in both endemic countries or when introduced into a formally FMD free country. Vaccines that protect against clinical disease and virus shedding are critical to control FMD. The replication deficient human adenovirus serotype 5 (Ad5) vaccine vector expressing empty FMD virus (FMDV) capsid, AdtFMD, is a promising new vaccine platform. With no shedding or spreading of viral vector detected in field trials, this vaccine is very safe to manufacture, as there is no requirement for high containment faciitites. Here, we describe three studies assessing the proportion of animals protected from clinical vesicular disease (foot lesions) following live-FMDV challenge by intradermolingual inoculation at 6 or 9 months following a single vaccination with the commercial AdtFMD vaccine, provisionally licensed for cattle in the United States. Further, we tested the effect of vaccination route (transdermal, intramuscular, subcutaneous) on clinical outcome and humoral immunity. Results demonstrate that a single dose vaccination in cattle with the commercial vaccine vector expressing capsid proteins of the FMDV strain A24 Cruzeiro (Adt.A24), induced protection against clinical FMD at 6 months (100% transdermal, 80% intramuscular, and 60% subcutaneous) that waned by 9 months post-vaccination (33% transdermal and 20% intramuscular). Post-vaccination serum from immunized cattle (all studies) generally contained FMDV specific neutralizing antibodies by day 14. Anti-FMDV antibody secreting cells are detected in peripheral blood early following vaccination, but are absent after 28 days post-vaccination. Thus, the decay in antibody mediated immunity over time is likely a function of FMDV-specific antibody half-life. These data reveal the short time span of anti-FMDV antibody secreting cells (ASCs) and important performance characteristics of needle-free vaccination with a recombinant vectored subunit vaccine for FMDV.