Cell interactions and cytotoxic studies of cellulose nanofibers from Curauá natural fibers. (1st December 2018)

• Record Type: Journal Article
• Authors: Souza, Sivoney Ferreira; Mariano, Marcos; Reis, Dennys; Lombello, Christiane Bertachini; Ferreira, Mariselma; Sain, Mohini
• Is Part Of: Carbohydrate polymers. Volume 201(2018)
• Page Start: 87
• Page End: 95
• Publication Date: 2018-12-01
• Subjects: Nanocellulose -- Nanofibrillated cellulose -- Microfibrillated cellulose -- Tensile strength -- Rheology -- Biocompatibility; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2018.08.056
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: Curauá fiber is an excellent candidate for nanocellulose obtainment due to its high initial cellulose content. Curauá fibers required mild chemical conditions and lower number of grinding cycles to produce cellulose nanofibers. SAXS technique revealed that cellulose nanofibers obtained from a mechanical process looks as a twisted ribbon. Cellulose Nanofibers from Curauá presented no cytotoxicity behavior for direct and indirect assays. Vero cells presented strong adhesion onto cellulose nanofiber film. Abstract: Cellulose nanofibers (CNF) were isolated from Curauá fibers ( Ananas erectifolius L. B. Smith ) through a mechanical grinder preceded by mild chemical treatment. Morphology and surface characteristics of the fibers were followed until it reaches the nanoscale as long and flexible nanofibers. In aqueous suspensions, SAXS techniques revealed that such nanofibers present a twisted ribbon structure while rheological measurements demonstrate its high viscosity and a thixotropic behavior. These characteristics suggests the potential application of CNF in biomedical field, which, in turn, stimulates the toxicological studies of such materials. The obtained materials do not show any sign of cytotoxicity by direct or indirect assays for cell viability and cell morphology using Vero cells. Moreover, during the adhesion test, the cells demonstrated higher affinity to the CNF surface. It can be related to its surface properties and its obtaining conditions, which did not use any hazardous chemicals.