Ammonia-oxidizing bacteria dominate ammonia oxidation in a full-scale wastewater treatment plant revealed by DNA-based stable isotope probing. (May 2018)

• Record Type: Journal Article
• Authors: Pan, Kai-Ling; Gao, Jing-Feng; Li, Hong-Yu; Fan, Xiao-Yan; Li, Ding-Chang; Jiang, Hao
• Is Part Of: Bioresource technology. Volume 256(2018)
• Page Start: 152
• Page End: 159
• Publication Date: 2018-05
• Subjects: Ammonia-oxidizing archaea -- Ammonia-oxidizing bacteria -- Seasonality -- Treatment process -- DNA-based stable isotope probing; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2018.02.012
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: DNA-SIP revealed AOB were more active than AOA during nitrification in test WWTP. Treatment process, not seasonality, varied AOB communities. AOA slightly outnumbered AOB in most of the samples. Nitrosomonadaceae -like AOB and Nitrosomonas spp. dominated active AOB. Most of the active AOA affiliated to Nitrososphaera viennensis . Abstract: A full-scale wastewater treatment plant (WWTP) with three separate treatment processes was selected to investigate the effects of seasonality and treatment process on the community structures of ammonia-oxidizing archaea (AOA) and bacteria (AOB). And then DNA-based stable isotope probing (DNA-SIP) was applied to explore the active ammonia oxidizers. The results of high-throughput sequencing indicated that treatment processes varied AOB communities rather than AOA communities. AOA slightly outnumbered AOB in most of the samples, whose abundance was significantly correlated with temperature. DNA-SIP results showed that the majority of AOB amoA gene was labeled by 13 C-substrate, while just a small amount of AOA amoA gene was labeled. As revealed by high-throughput sequencing of heavy DNA, Nitrosomonadaceae -like AOB, Nitrosomonas sp. NP1, Nitrosomonas oligotropha and Nitrosomonas marina were the active AOB, and Nitrososphaera viennensis dominated the active AOA. The results indicated that AOB, not AOA, dominated active ammonia oxidation in the test WWTP.