TC26, a teleost TFPI-1 derived antibacterial peptide that induces degradation of bacterial nucleic acids and inhibits bacterial infection in vivo. Issue 98 (March 2020)

• Record Type: Journal Article
• Authors: Su, Yan-li; Wang, Guang-hua; Wang, Jing-jing; Xie, Bing; Gu, Qin-qin; Hao, Dong-fang; Liu, Hong-mei; Zhang, Min
• Is Part Of: Fish & shellfish immunology. Issue 98(2020)
• Page Start: 508
• Page End: 514
• Publication Date: 2020-03
• Subjects: Common carp -- TFPI-1 -- Antibacterial peptide -- Antibacterial mechanism; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2020.01.057
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: At present, several reports have indicated that the C-terminal peptides of tissue factor pathway inhibitor 1 (TFPI-1) were active antibacterial peptides. However, the functions of TFPI-1 C-terminal peptides in teleost are still very limited. In this study, a C-terminal peptide, TC26 (with 26 amino acids), derived from common carp ( Cyprinus carpio ) TFPI-1, was synthesized and investigated for its antibacterial spectrum, action mechanism, as well as the in vivo effects on bacterial invasion. Our results showed that TC26 was active against Gram-positive bacteria Micrococcus luteus and Staphylococcus aureus, as well as Gram-negative bacterium Vibrio vulnificus . TC26 treatment facilitated the bactericidal process of erythromycin by enhancing the out-membrane permeability of V. vulnificus . During the bactericidal process, TC26 killed the target bacterial cells Vibrio vulnificus, by destroying cell membrane integrity, penetrating into the cytoplasm and inducing degradation of genomic DNA and total RNA. In vivo study showed that administration of turbot with TC26 before bacterial infection significantly reduced pathogen dissemination and replication in tissues. These results indicated that TC26 is a novel and active antibacterial peptide and may play a vital role in fighting pathogenic infection in aquaculture. Highlights: A common carp TFPI-1 peptide TC26 was bactericidal against Gram-positive and Gram-negative bacteria. TC26 treatment facilitated the bactericidal process of erythromycin. TC26 destroyed cell membrane integrity, penetrated into the cytoplasm, and induced degradation of nucleic acids. TC26 could inhibit bacterial invasion in turbot.