Stability of fungal biomass continuously fed with tannic acid in a non-sterile moving-packed bed reactor. (1st October 2019)

• Record Type: Journal Article
• Authors: Bardi, A.; Yuan, Q.; Siracusa, G.; Becarelli, S.; Di Gregorio, S.; Tigini, V.; Levin, D.B.; Petroni, G.; Munz, G.
• Is Part Of: Journal of environmental management. Volume 247(2019)
• Page Start: 67
• Page End: 77
• Publication Date: 2019-10-01
• Subjects: Bioreactors -- Immobilized biomass -- Non-sterile conditions -- Tannic acid -- White-rot fungi; Environmental policy -- Periodicals; Environmental management -- Periodicals; Environment -- Periodicals; Ecology -- Periodicals; 363.705
• DOI: 10.1016/j.jenvman.2019.06.036
• Languages: English
• ISSNs: 0301-4797

Abstract

Abstract: A number of bacteria and fungi are known to degrade tannins. In this study, the efficiency of the white-rot fungus, Bjerkandera adusta MUT 2295, was evaluated for the treatment of a synthetic solution prepared with tannic acid. Tests were performed in continuously fed, bench-scale, packed-bed reactors, operated under non-sterile conditions with biomass immobilized within PolyUrethane Foam cubes (PUFs). The main parameters monitored to evaluate the process efficiency were: soluble Chemical Oxygen Demand (sCOD), Total Organic Carbon (TOC) removal, and activities. of Tannase and Lignin Peroxidase. At the end of the process, additional parameters were evaluated, including the increase of fungal dry weight and the presence of ergosterol. The reactor was operative for 210 days, with maximum sCOD and TOC removal of 81% and 73%, respectively. The reduction of sCOD and TOC were positively correlated with the detection of Tannase and Lignin Peroxidase (LiP) activities. Increases in biomass within the PUF cubes was associated with increases in ergosterol concentrations. This study proved that the fungal-based system tested was efficient for the degradation of tannic acid over a period of time, and under non-sterile conditions. Graphical abstract: Image 1 Highlights: Tannic acid was treated in a continuously-fed reactor using a white-rot fungus. The reactor was operated for 210 days under non-sterile conditions. Depletion of the stable recalcitrant compound was achieved after 164 days. The process was associated with the detection of oxidative enzymes. Fungal treatment for remediation of tannic acid was demonstrated to be feasible.