Codelivery of Antigens and Adjuvant in Polymeric Nanoparticles Coated With Native Parasite Membranes Induces Protective Mucosal Immunity Against Giardia lamblia. (9th March 2022)

• Record Type: Journal Article
• Authors: Zhou, Jiarong; Miyamoto, Yukiko; Ihara, Sozaburo; Kroll, Ashley V; Nieskens, Noelle; Tran, Vivien N; Hanson, Elaine M; Fang, Ronnie H; Zhang, Liangfang; Eckmann, Lars
• Is Part Of: Journal of infectious diseases. Volume 226:Number 2(2022)
• Page Start: 319
• Page End: 323
• Publication Date: 2022-03-09
• Subjects: protozoa -- giardiasis -- intestinal mucosa -- nanovaccine -- biomimetic nanoparticle -- multivalent vaccine; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jiac074
• Languages: English
• ISSNs: 0022-1899

Abstract

Abstract: The protozoan pathogen Giardia lamblia is an important worldwide cause of diarrheal disease and malabsorption. Infection is managed with antimicrobials, although drug resistance and treatment failures are a clinical challenge. Prior infection provides significant protection, yet a human vaccine has not been realized. Individual antigens can elicit partial protection in experimental models, but protection is weaker than after prior infection. Here, we developed a multivalent nanovaccine by coating membranes derived from the parasite onto uniform and stable polymeric nanoparticles loaded with a mucosal adjuvant. Intranasal immunization with the nanovaccine induced adaptive immunity and effectively protected mice from G. lamblia infection. Abstract : A multivalent nanovaccine was fabricated by wrapping adjuvant-loaded polymeric nanoparticles with membranes derived from the protozoan pathogen Giardia lamblia . Mucosal immunization with the nanovaccine elicited robust adaptive immunity and protection against G. lamblia infection in mice.