Reliability of Self-Sampling for Accurate Assessment of Respiratory Virus Viral and Immunologic Kinetics. (25th July 2020)

• Record Type: Journal Article
• Authors: Waghmare, Alpana; Krantz, Elizabeth M; Baral, Subhasish; Vasquez, Emma; Loeffelholz, Tillie; Chung, E Lisa; Pandey, Urvashi; Kuypers, Jane; Duke, Elizabeth R; Jerome, Keith R; Greninger, Alexander L; Reeves, Daniel B; Hladik, Florian; Cardozo-Ojeda, E Fabian; Boeckh, Michael; Schiffer, Joshua T
• Is Part Of: Journal of infectious diseases. Volume 226:Number 2(2022)
• Page Start: 278
• Page End: 286
• Publication Date: 2020-07-25
• Subjects: respiratory virus -- viral diagnostics -- cytokines -- immune response; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jiaa451
• Languages: English
• ISSNs: 0022-1899

Abstract

Abstract: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic demonstrates the need for accurate and convenient approaches to diagnose and therapeutically monitor respiratory viral infections. We demonstrated that self-sampling with mid-nasal foam swabs is well-tolerated and provides quantitative viral output concordant with flocked swabs. Using longitudinal home-based self-sampling, we demonstrate that nasal cytokine levels correlate and cluster according to immune cell of origin. Periods of stable viral loads are followed by rapid elimination, which could be coupled with cytokine expansion and contraction. Nasal foam swab self-sampling at home provides a precise, mechanistic readout of respiratory virus shedding and local immune responses. Abstract : Self-sampling with foam swabs is well-tolerated and provides quantitative viral output concordant with flocked swabs. Using longitudinal home-based self-sampling, we demonstrate that nasal foam swab sampling provides a precise, mechanistic readout of respiratory virus shedding and local immune responses.