A novel methanotrophic co-metabolic system with high soluble methane monooxygenase activity to biodegrade refractory organics in pulping wastewater. (May 2018)

• Record Type: Journal Article
• Authors: Li, Yancheng; Wang, Yingmu; Lin, Ziyuan; Wang, Jiale; He, Qiang; Zhou, Jian
• Is Part Of: Bioresource technology. Volume 256(2018)
• Page Start: 358
• Page End: 365
• Publication Date: 2018-05
• Subjects: Pulping wastewater -- Refractory organics -- Methanotrophs -- Co-metabolic system -- sMMO; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2018.02.048
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: A methanotrophic co-metabolic system was built in gcSBBR. Refractory organics in pulping wastewater was effectively biodegraded. This co-metabolic system possessed high sMMO activity and contained gene mmoX. Methylocaldum, Methylococcus and Methylomonas detected belonged to methanotrophs. A postulated synergy pathway to biodegrade refractory organics was put forward. Abstract: Pulping wastewater still contains massive refractory organics after biotreatment, with high colority, low biodegradability, and lasting biotoxicity. To eliminate refractory organics in pulping wastewater, a methanotrophic co-metabolic system in a gas cycle Sequencing Batch Biofilm Reactor (gcSBBR) seeded by soil at a ventilation opening of coal mine was quickly built on the 92nd day. The removal rate of COD, colority and TOC was 53.28%, 50.59% and 51.60%, respectively. Analysis of 3D-EEM indicated that glycolated protein-like, melanoidin-like or lignocellulose-like, and humic acid-like decreased by 7.85%, 5.02% and 1.74%, respectively. Moreover, this system exhibited high activity of soluble methane monooxygenase (sMMO) and mmoX encoding sMMO reached up to 7.89 × 10 5 copies/μL. Methanotrophs, namely, Methylocaldum (8.28%), Methylococcus (6.06%) and Methylomonas (0.07%), were detected by 16S rRNA sequencing. And other bacteria were dominated by Denitratisoma, Anaerolineaceae _uncultured and Methylophilaceae _uncultured. Refractory organics was biodegraded through the synergy among microorganisms, and a postulated synergy pathway was put forward.