Freezing in the presence of nitrite pretreatment enhances hydrogen production from dark fermentation of waste activated sludge. (1st March 2020)

• Record Type: Journal Article
• Authors: Liu, Xuran; He, Dandan; Wu, Yanxin; Xu, Qiuxiang; Wang, Dongbo; Yang, Qi; Liu, Yiwen; Ni, Bing-Jie; Wang, Qilin; Li, Xiaoming
• Is Part Of: Journal of cleaner production. Volume 248(2020)
• Publication Date: 2020-03-01
• Subjects: Waste activated sludge -- Dark fermentation -- Hydrogen -- Nitrite -- Freezing treatment; Factory and trade waste -- Management -- Periodicals; Manufactures -- Environmental aspects -- Periodicals; Déchets industriels -- Gestion -- Périodiques; Usines -- Aspect de l'environnement -- Périodiques; 628.5
• DOI: 10.1016/j.jclepro.2019.119305
• Languages: English
• ISSNs: 0959-6526

Abstract

Abstract: Due to the poor biodegradability of released organics and the rapid consumption of hydrogen, hydrogen production from the untreated waste activated sludge (WAS) and/or inocula is still limited. In this study, it was found that the dark fermentative hydrogen production was largely enhanced from WAS pretreated by freezing in the presence of nitrite. With an increase of nitrite addition from 100 to 400 mg NO2 − -N/L during freezing pretreatment (−5 °C for 4 h), the maximal hydrogen yield increased from 7.96 to 19.40 mL/g VS (volatile solids), which was 5.5–13.4 times of that in the control (without freezing and nitrite addition). Mechanism explorations revealed that the proposed pretreatment not only accelerated the disintegration of sludge but also promoted the proportion of biodegradable organics released, thereby provided more bio-available substrates for subsequent hydrogen production. Proposed pretreatment severely suppressed the sludge microorganisms responding to homoacetogenesis (−32.1%), methanogenesis (−58.4%), and sulfate-reducing process (−51.5%), inhibited the consumption of hydrogen. Moreover, there was more acetic and butyric (76% versus 57.5%) but less propionic acid (22.6% versus 13.4%) in this pretreated fermenter, which was in correspondence with the theory of fermentation type affecting hydrogen production. Long-term fermentation experiments indicated that the proposed pretreatment boosted the [FeFe]-hydrogenase activities while suppressed the activities of carbon monoxide dehydrogenase, coenzyme F420, and adenylyl sulfate reductase. Graphical abstract: Image 1 Highlights: FNP enhanced dark fermentative hydrogen production from WAS by 5.5–13.4 times. FNP accelerated sludge disintegration and promoted organics biodegradability. FNP suppressed the hydrogen-consuming bacteria by 32.1–58.4%. FNP induced the shift of fermentation to butyrate-type.