Inhibition of lipid accumulation by the ethyl acetate fraction of Distylium racemosum in vitro and in vivo. (2019)

• Record Type: Journal Article
• Authors: Kim, Hye-Ran; Jung, Bo-Kyoung; Yeo, Min-Ho; Yoon, Weon-Jong; Chang, Kyung-Soo
• Is Part Of: Toxicology reports. Volume 6(2019)
• Page Start: 215
• Page End: 221
• Publication Date: 2019
• Subjects: Obesity -- Distylium racemosum -- Ethyl acetate fraction; Toxicology -- Periodicals; Clinical toxicology -- Periodicals; Drug-Related Side Effects and Adverse Reactions; Hazardous Substances; Poisoning; Toxicology; Electronic journals; Periodicals; Periodicals; 571.9505
• DOI: 10.1016/j.toxrep.2019.02.003
• Languages: English
• ISSNs: 2214-7500

Abstract

Highlights: Lipid accumulation in the 3T3-L1 cells were inhibited by treatment with DRE. The expression levels of SREBP1c, PPARγ, C/EBPα, and FAS were decreased by DRE. HFD induced fat mice showed lower rate of weight gain and serum TG level through DRE administration. Abstract: This study confirms the anti-obesity effect of the ethyl acetate fraction of Distylium racemosum (DRE), a member of Hamamelidaceae, that naturally grows on Jeju Island, on adipocyte differentiation in 3T3-L1 cells. This study further demonstrated that DRE exhibits anti-obesity effects in C57BL/6 obese mice. The degree of adipocyte differentiation was determined using Oil red O stain; results indicated a decrease in fat globules, which was dependent on DRE concentration, when pre-adipocytes were treated with differentiation-inducing agents. In addition, this significantly reduced the expression of the adipogenic transcription factor and related genes. C57BL/6 obese mice treated with DRE showed a lower rate of body weight gain than the high-fat diet (HFD) group mice. Further, the level of serum triglyceride in the DRE treatment group was lower than that in the HFD group. The findings show that DRE are capable of suppressing adipocyte accumulation; therefore, DRE may represent a promising source of functional materials for the anti-obesity.