Development of a multi-stage continuous fermentation strategy for docosahexaenoic acid production by Schizochytrium sp. (December 2018)

• Record Type: Journal Article
• Authors: Guo, Dong-Sheng; Ji, Xiao-Jun; Ren, Lu-Jing; Yin, Feng-Wei; Sun, Xiao-Man; Huang, He; Zhen, Gao
• Is Part Of: Bioresource technology. Volume 269(2018)
• Page Start: 32
• Page End: 39
• Publication Date: 2018-12
• Subjects: Docosahexaenoic acid -- Schizochytrium sp. -- Continuous fermentation -- Kinetic modeling; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2018.08.066
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Fermentation kinetics of DHA production using Schizochytrium sp. was developed. Growth-uncoupled DHA production by Schizochytrium sp. was studied. Multi-stage continuous fermentation prediction model was established and applied. The DHA efficient production was realized by multi-stage continuous fermentation. Abstract: Docosahexaenoic acid (DHA) has wide-ranging benefits for normal development of the visual and nervous systems in infants. A sustainable source of DHA production through fermentation using Schizochytrium sp. has been developed. In this paper, we present the discovery of growth-uncoupled DHA production by Schizochytrium sp. and the development of corresponding kinetic models of fed-batch fermentations, which can be used to describe and predict the cell growth and substrate utilization as well as lipid and DHA production. Based on this kinetic model, a predictive model of multi-stage continuous fermentation process was established and used to analyze, optimize and design the process parameters. Optimal predicted processes of two-stage and three-stage continuous fermentation were developed and verified in lab-scale bioreactor based on the predicted process parameters. A successful three-stage continuous fermentation was achieved, which increased the lipid, DHA content and DHA productivity by 47.6, 64.3 and 97.1%, respectively, compared with two-stage continuous fermentation.