Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence. (February 2020)

• Record Type: Journal Article
• Authors: Ramírez, Laura; de Moura, Luana Dias; Mateus, Natalia Lopes Fontoura; de Moraes, Milene Hoehr; do Nascimento, Leopoldo Fabrício Marçal; de Jesus Melo, Nailson; Taketa, Lucas Bezerra; Catecati, Tatiana; Huete, Samuel G.; Penichet, Karla; Piranda, Eliane Mattos; de Oliveira, Alessandra Gutierrez; Steindel, Mario; Barral-Netto, Manoel; do Socorro Pires e Cruz, Maria; Barral, Aldina; Soto, Manuel
• Is Part Of: Parasite epidemiology and control. Volume 8(2020)
• Publication Date: 2020-02
• Subjects: BB blocking buffer -- CanL Canine visceral leishmaniasis -- EDCB ELISA denaturant coating buffer -- ELISA enzyme-linked immunosorbent assay -- HSP Heat shock protein -- KMP-11 Kinetoplastid-membrane protein of 11 kDa -- LR Likelihood ratio -- MS Mato Grosso do Sul State (Brazil) -- PBS phosphate saline buffer -- PI Piaui State (Brazil) -- ROC Receiver Operating Characteristic -- RR Relative reactivity -- RT Room temperature -- SC Santa Catarina State (Brazil) -- SLA Soluble leishmanial antigen -- VL Visceral leishmaniosis -- WB Washing buffer; Leishmania -- Canine leishmaniasis -- Serodiagnosis -- Antibodies -- Recombinant proteins; Parasitic diseases -- Epidemiology -- Periodicals; Parasitic diseases -- Prevention -- Periodicals; Parasitology -- Periodicals; Parasitic Diseases; Parasitic diseases -- Epidemiology; Parasitic diseases -- Prevention; Parasitology; Periodicals; Periodicals; 571.99905
• DOI: 10.1016/j.parepi.2019.e00126
• Languages: English
• ISSNs: 2405-6731

Abstract

Abstract: Serodiagnosis of Leishmania infantum infection in dogs relies on the detection of antibodies against leishmanial crude extracts or parasitic defined antigens. The expansion of canine leishmaniasis from geographical areas of Brazil in which the infection is endemic to regions in which the disease is emerging is occurring. This fact makes necessary the analysis of the serodiagnostic capabilities of different leishmanial preparations in distinct geographical locations. In this article sera from dogs infected with Leishmania and showing the clinical form of the disease, were collected in three distinct Brazilian States and were tested against soluble leishmanial antigens or seven parasite individual antigens produced as recombinant proteins. We show that the recognition of soluble leishmanial antigens by sera from these animals was influenced by the geographical location of the infected dogs. Efficacy of the diagnosis based on this crude parasite preparation was higher in newly endemic regions when compared with areas of high disease endemicity. We also show that the use of three of the recombinant proteins, namely parasite surface kinetoplastid membrane protein of 11 kDa (KMP-11), and two members of the P protein family (P2a and P0), can improve the degree of sensitivity without adversely affecting the specificity of the diagnostic assays for canine leishmaniasis, independently of the geographical area of residence. In addition, sera from dogs clinically healthy but infected were also assayed with some of the antigen preparations. We demonstrate that the use of these proteins can help to the serodiagnosis of Leishmania infected animals with subclinical infections. Finally, we propose a diagnostic protocol using a combination of KMP-11, P2a y P0, together with total leishmanial extracts. Graphical abstract: Unlabelled Image Highlights: Serodiagnosis of canine leishmaniasis based on SLA is compromised in regions of high endemicity. The use of individual antigens improves serodiagnosis of canine leishmaniasis. Sera from clinically ill and subclinically infected dogs possess specific antibodies for KMP-11, LiP2a or LiP0 proteins. The best criterion of positivity is to have reactivity for at least one of the next antigens: SLA, KMP-11, LiP2a, LiP0.