One step selective partition of ε-polylysine present in broth cultures in ionic liquid-based aqueous biphasic systems. Issue 3 (11th February 2021)

• Record Type: Journal Article
• Authors: Sequeira, Rosy Alphons; Sharma, Mukesh; Pereira, Matheus M.; Singh, Nripat; Bhattacharya, Sourish; Chudasama, Nishith A; Prasad, Kamalesh
• Is Part Of: Separation science and technology. Volume 56:Issue 3(2021)
• Page Start: 631
• Page End: 639
• Publication Date: 2021-02-11
• Subjects: Ionic liquid -- ε-poly-L-lysine -- polyamide -- aqueous biphasic system -- separation; Separation (Technology) -- Periodicals; 660.284205
• DOI: 10.1080/01496395.2020.1728323
• Languages: English
• ISSNs: 0149-6395

Abstract

ABSTRACT: Four different ionic liquids were synthesized, characterized and used with polypropylene glycol for the formation of aqueous biphasic systems and were used to selectively separate ε-PL from culture broth. It was observed that the partition of ε-PL to the IL-rich phase was achieved, and with pure ε-PL, the extraction efficiency was found to be 99.92% while with culture broth, it was 93.42% for the ABS formed with 2-hydroxyethylammonium formate. Preservation of the chemical and structural stability of ε-PL in the ABSs indicated the potential of such systems as remarkable platform for the selective separation of ε-PL from the culture broth.