Intracellular Delivery of Budesonide and Polydopamine Co‐Loaded in Endosomolytic Poly(butyl methacrylate‐co‐methacrylic acid) Grafted Acetalated Dextran for Macrophage Phenotype Switch from M1 to M2. Issue 1 (14th May 2020)

• Record Type: Journal Article
• Authors: Wang, Shiqi; Wannasarit, Saowanee; Figueiredo, Patrícia; Molinaro, Giuseppina; Ding, Yaping; Correia, Alexandra; Casettari, Luca; Wiwattanapatapee, Ruedeekorn; Hirvonen, Jouni; Liu, Dongfei; Li, Wei; Santos, Hélder A.
• Is Part Of: Advanced therapeutics. Volume 4:Issue 1(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-05-14
• Subjects: drug delivery -- endosomal escape -- macrophage phenotypes -- microfluidics -- polydopamine; Therapeutics -- Periodicals; Pharmaceutical technology -- Periodicals; Pharmacogenetics -- Periodicals; 615.5
• DOI: 10.1002/adtp.202000058
• Languages: English
• ISSNs: 2366-3987

Abstract

Abstract: In this study, a rationally designed nanocomposite (BUDPDA@MAP) composed of polydopamine (PDA) nanoparticle and anti‐inflammatory drug budesonide (BUD) encapsulated in a pH‐responsive endosomolytic polymer (poly(butyl methacrylate‐ co ‐methacrylic acid) grafted acetalated dextran, denoted by MAP), is proposed. The uniform nanocomposite is prepared using a microfluidic device. At low endosomal pH (5.5), MAP destabilizes the endosomal membranes for the cytoplasmic delivery of PDA, and releases BUD simultaneously, resulting in a greater reactive oxygen species scavenging capability than both the free drug and PDA alone. The combined therapeutic efficacy from PDA and BUD also leads to a successful macrophage phenotype switch from pro‐inflammatory M1 to anti‐inflammatory M2. Abstract : A nanocomposite for the intracellular co‐delivery of polydopamine nanoparticle and budesonide is developed by a microfluidic technique. With endosomal escape capability and pH‐controlled drug release profile, the nanocomposite shows efficient reactive oxygen species scavenging properties and induces a successful macrophage phenotype switch in vitro.