Double‐Stranded DNA‐Templated Copper Nanoclusters for Detection of DNA Polymerase Activity. (13th November 2020)

• Record Type: Journal Article
• Authors: Kim, Jungeun; Gang, Jongback
• Is Part Of: Bulletin of the Korean Chemical Society. Volume 42:Number 1(2021)
• Page Start: 32
• Page End: 36
• Publication Date: 2020-11-13
• Subjects: DNA‐templated copper nanoclusters -- DNA‐CuNCs -- Biosensor -- DNA polymerase -- Klenow fragment; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/bkcs.12142
• Languages: English
• ISSNs: 0253-2964

Abstract

Abstract : DNA polymerase plays an important role in the proliferation and survival of all forms of life and the variety of biotechnological applications. In this study, DNA‐templated copper nanoclusters were utilized to detect Klenow fragment exo ‐ (KF‐) activity and monitor its inhibition in the presence of 5‐fluorouracil by measurement of fluorescence intensities. This study showed that the fluorescence intensities increased continuously for KF‐ concentrations ranging from 0 to 5 U/mL. In addition, inhibition of KF‐ activity was measured at 5‐fluorouracil concentrations ranging from 0 to 150 μM. Using nonlinear regression, the corresponding IC50 was determined to be 6.23 μM in the presence of 5.0 U/mL KF‐. Abstract : In this study, DNA‐templated copper nanoclusters (DNA‐CuNCs) were utilized to detect Klenow fragment exo‐ (KF‐) activity. This study showed that the fluorescence intensities increased continuously for KF‐ concentrations ranging from 0 to 5 U/mL. It indicated that DNA polymerization activity was detected using hairpin DNA‐templated copper nanoclusters with simplicity and efficiency.