Application of Neuron‐Selective Fluorescent Probe, NeuA, To Identify Mouse Retinal Degeneration. (25th March 2021)

• Record Type: Journal Article
• Authors: Park, Sung‐Jin; Woon, Queenie Tan Shu; Er, Jun Cheng; Wong, Bernice H.; Liu, Xiao; Kang, Nam‐Young; Barathi, Veluchamy A.; Silver, David L.; Chang, Young‐Tae
• Is Part Of: Chembiochem. Volume 22:Number 11(2021)
• Page Start: 1915
• Page End: 1919
• Publication Date: 2021-03-25
• Subjects: fluorescent probe -- NeuA -- outer segment of photoreceptors -- retinal degeneration -- retinal application; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.202100011
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: The retina is part of the central nerve system (CNS) and has various interneurons and sensory neurons such as photoreceptor cells. Retinitis pigmentosa (RP) is an inherited condition that is characterized by photoreceptor degeneration. Herein, we developed a fluorescent probe‐NeuA‐for detecting retinal neuronal cells and applied NeuA to discriminate between healthy and RP retinas. The staining pattern of NeuA in the retinas of healthy and RP mouse models was examined in vitro, ex vivo and in vivo using confocal microscopy, the fluorescent fundus microscopy and optical coherent tomography (OCT). NeuA strongly stained the outer segment layer of photoreceptor cells and some bipolar cells in the healthy retina, but there was only weak staining in the photoreceptor degenerated retinas. Therefore, NeuA probe can be used as the detecting RP tools in the preclinical conditions. Abstract : As far as the eye can be seen : Developed fluorescent probe, NeuA (NeuO with an acrylamide group), stained healthy mouse retinal neuron cells and discriminated between healthy retina and retina with degenerating photoreceptors by confocal and fluorescent fundus microscopy. These imaging probes can be used as diagnostic and prognostic tools for retinitis pigmentosa in a clinical domain.