Application of induced pluripotent stem cell and embryonic stem cell technology to the study of male infertility. Issue 11 (5th June 2018)

• Record Type: Journal Article
• Authors: Mahabadi, Javad Amini; Sabzalipour, Hamed; Bafrani, Hassan Hassani; Gheibi Hayat, Seyed Mohammad; Nikzad, Hossein
• Is Part Of: Journal of cellular physiology. Volume 233:Issue 11(2018:Nov.)
• Page Start: 8441
• Page End: 8449
• Publication Date: 2018-06-05
• Subjects: azoospermia -- embryonic stem cell -- induced pluripotent stem cell -- male infertility -- testicular cancer; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.26757
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract : Stem cells (SCs) are classes of undifferentiated biological cells existing only at the embryonic, fetal, and adult stages that can divide to produce specialized cell types during fetal development and remain in our bodies throughout life. The progression of regenerative and reproductive medicine owes the advancement of respective in vitro and in vivo biological science on the stem cell nature under appropriate conditions. The SCs are promising therapeutic tools to treat currently of infertility because of wide sources and high potency to differentiate. Nevertheless, no effective remedies are available to deal with severe infertility due to congenital or gonadotoxic stem cell deficiency in prepubertal childhood. Some recent solutions have been developed to address the severe fertility problems, including in vitro formation of germ cells from stem cells, induction of pluripotency from somatic cells, and production of patient‐specific pluripotent stem cells. There is a possibility of fertility restoration using the in vitro formation of germ cells from somatic cells. Accordingly, the present review aimed at studying the literature published on the medical application of stem cells in reproductive concerns. Abstract : Sources of cells from spermatogonia stem cells can be derived. (A) Isolated pluripotent inner mass cells are obtained from donated fertilization embryos, which are at the blastocyst stage, followed by indefinite expansion in culture as human embryonic stem cells. (B) As an alternative way, patient‐specific induced pluripotent stem cells are able to be reprogrammed from somatic cells, such as fibroblasts, by transduction using a cocktail of embryonic transcription factors (sex‐determining region Y‐box 2, octamer‐binding transcription factor 4, c‐Myc, c‐myc, and Kruppel‐like factor 4).