Polyethylenimine: A new differentiation factor to endothelial/cardiac tissue. Issue 2 (1st September 2018) - Hosseinzadeh, Simzar
Abstract: Among different tissues, endothelial/cardiac types require specific factors to promote myocardial regeneration after occurred injuries. Herein, cardiac stem cells (CSCs) as the major cell population that involved in cardiovascular repair were selected to study the role of polyethyleneimine (PEI) agent on endothelial differentiation. After preparation of electrospun network of PEI with polyacrylonitrile, the related characterizations were carried out including scanning electron microscope (SEM), field‐emission SEM, water contact angle, Fourier transform infrared spectroscopy and mechanical properties. Also, the release kinetic of the corresponding agent was studied up to 7 days. The cell differentiation studies were done in the following with 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyl tetrazolium bromide assay, Real‐time polymerase chain reaction and immunostaining method. The whole obtained results approved the higher differentiation of CSCs into endothelial/cardiac cells. Finally, it is recommended that the PEI delivering increases the healing potency of CSCs and accordingly the regeneration speed of damaged cardiovascular tissue would be improved. Abstract : 1. Vascularization is the main occurrence in tissue engineering of different tissues. 2. Chemical factors as vascular endothelial growth factor could be replaced with polymeric substitute materials. 3. Polyethylenimine (PEI) can mimic the role of angiogenic agents as arginine, putrescine, spermidine, and spermine. 4. The electrospun scaffold with control release of PEI led cardiac progenitor cells (cardiac stem cells) differentiation into endothelial/cardiac cells.