Evaluation of osteoblast differentiation and function when cultured on mesoporous bioactive glass adsorbed with testosterone. Issue 7 (12th March 2018)

• Record Type: Journal Article
• Authors: Gao, Kai; Wang, Xiaoyan; Liu, Qianqian; Chen, Wei; Wang, Gan; Zhang, Dongyi; Liu, Long
• Is Part Of: Journal of cellular biochemistry. Volume 119:Issue 7(2018)
• Page Start: 5222
• Page End: 5232
• Publication Date: 2018-03-12
• Subjects: Erk1/2‐Runx2 pathway -- MBG/T -- MC3T3‐E1 -- testosterone; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.26566
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract: Mesoporous bioactive glass (MBG), a kind of porous materials with great osteoconductive and osteoinductive ability, shows promising application in bone tissue engineering due to its high specific surface area, orderly channel structure, and large pore volume. Here we reported that the proliferation, differentiation, and mineralization were promoted in MC3T3‐E1 cells cultured on MBG which adsorbed with testosterone (MBG/T). We found that transcriptional activity of Runx2 which is a critical transcription factor is increased in MC3T3‐E1 cells cultured on MBG/T. Intriguingly, we observed that ERK phosphorylation was enhanced in MC3T3‐E1 cells cultured on MBG/T. We showed that activated Runx2 in MC3T3‐E1 cells cultured on MBG/T is through Erk1/2 phosphorylation. Consistent with this result, we also found that the expression of osteoblastic marker genes were increased. Therefore, we concluded that osteoblast differentiation and mineralization was enhanced after cells cultured on MBG/T through Erk1/2‐activated Runx2 pathway. Our findings provided that MBG/T is a potential material in the process of bone repair. Abstract : The osteogenetic performance brought by MBG/T make a promising material for repairing bone defects related disease.