Growth Kinetics Monitoring of Gram-Negative Pathogenic Microbes Using Raman Spectroscopy. Issue 10 (October 2022)

• Record Type: Journal Article
• Authors: Saikia, Dimple; Jadhav, Priyanka; Hole, Arti R.; Krishna, Chilakapati Murali; Singh, Surya P.
• Is Part Of: Applied spectroscopy. Volume 76:Issue 10(2022)
• Page Start: 1263
• Page End: 1271
• Publication Date: 2022-10
• Subjects: Raman spectroscopy -- biomedical optics -- microbes -- principal component analysis -- PCA; Spectrum analysis -- Periodicals; 543.505
• DOI: 10.1177/00037028221109624
• Languages: English
• ISSNs: 0003-7028

Abstract

Optical density based measurements are routinely performed to monitor the growth of microbes. These measurements solely depend upon the number of cells and do not provide any information about the changes in the biochemical milieu or biological status. An objective information about these parameters is essential for evaluation of novel therapies and for maximizing the metabolite production. In the present study, we have applied Raman spectroscopy to monitor growth kinetics of three different pathogenic Gram-negative microbes Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumannii . Spectral measurements were performed under 532 nm excitation with 5 seconds of exposure time. Spectral features suggest temporal changes in the "peptide" and "nucleic acid" content of cells under different growth stages. Using principal component analysis (PCA), successful discrimination between growth phases was also achieved. Overall, the findings are supportive of the prospective adoption of Raman based approaches for monitoring microbial growth. Graphical Abstract