Silicon induces adventitious root formation in rice under arsenate stress with involvement of nitric oxide and indole-3-acetic acid. (24th May 2021)

• Record Type: Journal Article
• Authors: Tripathi, Durgesh Kumar; Rai, Padmaja; Guerriero, Gea; Sharma, Shivesh; Corpas, Francisco J; Singh, Vijay Pratap
• Editors: Cuypers, Ann
• Is Part Of: Journal of experimental botany. Volume 72:Number 12(2021)
• Page Start: 4457
• Page End: 4471
• Publication Date: 2021-05-24
• Subjects: Arsenic -- ascorbate -- auxin -- cell cycle dynamics -- cell viability -- gene expression -- nitric oxide -- rice -- silicon; Botany -- Periodicals; Botany, Experimental -- Periodicals; Plant physiology -- Periodicals; 580
• DOI: 10.1093/jxb/eraa488
• Languages: English
• ISSNs: 0022-0957

Abstract

Abstract: Arsenic (As) negatively affects plant development. This study evaluates how the application of silicon (Si) can favor the formation of adventitious roots in rice under arsenate stress (As V ) as a mechanism to mitigate its negative effects. The simultaneous application of As V and Si up-regulated the expression of genes involved in nitric oxide (NO) metabolism, cell cycle progression, auxin (IAA, indole-3-acetic acid) biosynthesis and transport, and Si uptake which accompanied adventitious root formation. Furthermore, Si triggered the expression and activity of enzymes involved in ascorbate recycling. Treatment with L-NAME ( N G -nitro L-arginine methyl ester), an inhibitor of NO generation, significantly suppressed adventitious root formation, even in the presence of Si; however, supplying NO in the growth media rescued its effects. Our data suggest that both NO and IAA are essential for Si-mediated adventitious root formation under As V stress. Interestingly, TIBA (2, 3, 5-triiodobenzoic acid), a polar auxin transport inhibitor, suppressed adventitious root formation even in the presence of Si and SNP (sodium nitroprusside, an NO donor), suggesting that Si is involved in a mechanism whereby a cellular signal is triggered and that first requires NO formation, followed by IAA biosynthesis. Abstract : Silicon requires nitric oxide and indole-3-acetic acid to promote adventitious root formation under arsenate stress in rice seedlings.