Dynamics of proteins in solution. (2019)

• Record Type: Journal Article
• Authors: Grimaldo, Marco; Roosen-Runge, Felix; Zhang, Fajun; Schreiber, Frank; Seydel, Tilo
• Is Part Of: Quarterly reviews of biophysics. Volume 52(2019)
• Publication Date: 2019
• Subjects: Quasielastic neutron spectroscopy, -- protein diffusion, -- protein backbone, -- protein side chains, -- molecular relaxations, -- colloid physics; Biophysics -- Periodicals; 571.405
• DOI: 10.1017/S0033583519000027
• Languages: English
• ISSNs: 0033-5835

Abstract

Abstract: The dynamics of proteins in solution includes a variety of processes, such as backbone and side-chain fluctuations, interdomain motions, as well as global rotational and translational (i.e. center of mass) diffusion. Since protein dynamics is related to protein function and essential transport processes, a detailed mechanistic understanding and monitoring of protein dynamics in solution is highly desirable. The hierarchical character of protein dynamics requires experimental tools addressing a broad range of time- and length scales. We discuss how different techniques contribute to a comprehensive picture of protein dynamics, and focus in particular on results from neutron spectroscopy. We outline the underlying principles and review available instrumentation as well as related analysis frameworks.