Effects of potential inhibitors present in dilute acid-pretreated corn stover on fermentative hydrogen production by Escherichia coli. (8th August 2022)

• Record Type: Journal Article
• Authors: Teramoto, Haruhiko; Suda, Masako; Inui, Masayuki
• Is Part Of: International journal of hydrogen energy. Volume 47:Number 68(2022)
• Page Start: 29219
• Page End: 29229
• Publication Date: 2022-08-08
• Subjects: Escherichia coli -- Fermentative hydrogen production -- Lignocellulosic biomass -- Dilute acid pretreatment -- Fermentation inhibitors; Hydrogen as fuel -- Periodicals; Hydrogène (Combustible) -- Périodiques; Hydrogen as fuel; Periodicals; 665.81
• DOI: 10.1016/j.ijhydene.2022.06.267
• Languages: English
• ISSNs: 0360-3199

Abstract

Abstract: This study reports the inhibitory compounds present in dilute sulfuric acid-pretreated corn stover for fermentative hydrogen production by Escherichia coli . The E. coli W3110-derived strain deficient in lactate and succinate production pathways was used. When dilute acid-treated corn stover liquor containing 175 mM acetate, 21 mM furfural, and 2.6 mM 5-hydroxymethylfurfural was added at fourfold dilution to the xylose-containing culture medium, the microbial growth and hydrogen production were almost completely inhibited. The addition of acetate, furfural, or 5-hydroxymethylfurfural, at up to 200, 80, or 40 mM, respectively, to the xylose- or glucose-containing medium led to dose-dependent inhibition of fermentative hydrogen production. No synergistic inhibition by furfural with acetate or 5-hydroxymethylfurfural at concentrations based on the corn stover hydrolysate was observed. Altogether, these results suggest that the inhibition of the E. coli -based hydrogen production by the corn stover hydrolysate is primarily attributed to acetate under the conditions used. Highlights: Corn stover is a promising feedstock for H2 production by Escherichia coli . New insights into fermentation inhibitors for bio-H2 production are obtained. Acetate is a critical inhibitor for Escherichia coli engineered for H2 production.