Degradation of β-lactam antibiotic ampicillin using sustainable microbial peroxide producing cell system. (October 2022)

• Record Type: Journal Article
• Authors: Mukhopadhyay, Dhruva; Khan, Nawaz; Kamal, Neha; Varjani, Sunita; Singh, Shivani; Sindhu, Raveendran; Gupta, Pratima; Bhargava, Preeti Chaturvedi
• Is Part Of: Bioresource technology. Volume 361(2022)
• Publication Date: 2022-10
• Subjects: β-Lactam antibiotics -- Microbial electro-fenton system -- Biodegradation -- Wastewater treatment; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.127605
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Microbial Peroxide Producing Cell assisted degradation of ampicillin was achieved. Electrochemical production of 36 ± 3.20 to 88 ± 2.90 mM H2 O2 was seen. Microbial electrofenton resulted into in-situ degradation of 96–98% of ampicillin. Degraded ampicillin was found inactive for sensitivity assay against E.coli. Simultaneous wastewater-treatment & mitigation of emerging contaminant was done. Abstract: The enormous use of synthetic antibiotic and personal care products has impacted the natural microbiome and ecosystem. Overtime, treatment technologies developed suffered due to incomplete removal hence, a pilot dual-chambered microbial peroxide-producing cell that degrades ampicillin catalyzed by homogenous Fenton-reaction was designed. The system reported maximum current at 16.714 ± 0.048 µAcm −2, power output of 1.956 ± 0.015 mW m −2 ; 88 ± 2.90 mM of H2 O2 generation with Na2 SO4 that degraded 95.9 ± 3.00 to 97.8 ± 3.20% of 10 mg L −1 ampicillin within 72 hrs with electro-active Shewanella putrefaciens. An E. coli bioactivity assay with ampicillin exhibited no sensitivity zone due to the loss of activity. Analytical spectroscopic studies reveal β-Lactam ring deformation; Liquid Chromatography-Mass Spectroscopy clearly shows the presence of degradation metabolites. A sustainable wastewater treatment with 72 ± 4.5% reduction in anodic chemical oxygen demand was achieved. Present results designate the technology, as promising for effective antibiotics removal for wastewater treatment concomitant with electricity generation.