Mapping of STB/HAP1 Immunoreactivity in the Mouse Brainstem and its Relationships with Choline Acetyltransferase, with Special Emphasis on Cranial Nerve Motor and Preganglionic Autonomic Nuclei. (1st September 2022)

• Record Type: Journal Article
• Authors: Islam, Md Nabiul; Miyasato, Emi; Jahan, Mir Rubayet; Tarif, Abu Md Mamun; Nozaki, Kanako; Masumoto, Koh-hei; Yanai, Akie; Shinoda, Koh
• Is Part Of: Neuroscience. Volume 499(2022)
• Page Start: 40
• Page End: 63
• Publication Date: 2022-09-01
• Subjects: Huntingtin-associated protein 1 -- Stigmoid body -- Motor neuron -- Autonomic neuron -- Neuroprotection -- ChAT; 2Cb second cerebellar lobule -- 3N oculomotor nuclei -- 4N trochlear nuclei -- 4V 4th ventricle -- 5N trigeminal motor nuclei -- 6N abducens nuclei -- 7N facial nuclei -- 7n facial nerve -- 10N dorsal motor nucleus of vagus -- 11N nucleus of the accessory nerve -- 12N nucleus of the hypoglossal nerve -- Amb nucleus ambiguus -- AP area postrema -- Aq aqueduct of Sylvius -- Bar Barrington's nucleus -- CC central canal -- CG central gray -- CLi caudal linear nucleus of raphe -- DCIC dorsal cortex inferior colliculus -- DpG deep gray layer of the superior colliculus -- DR dorsal raphe nuclei -- DRD dorsal raphe nucleus dorsal part -- DRI dorsal raphe nucleus interfascicular part -- DRL dorsal raphe nucleus lateral part -- DRV dorsal raphe nucleus ventral part -- ECIC external cortex of the inferior colliculus -- EW Edinger-Westphal nucleus -- EWcp Edinger-Westphal centrally projecting -- EWpg Edinger-Westphal preganglionic -- GFAP glial fibrillary acidic protein -- HAP1 huntingtin-associated protein 1 -- Iba1 ionized calcium binding adaptor molecule 1 -- IF interfascicular nucleus -- InG intermediate gray layer of the superior colliculus -- IP interpedicular nucleus -- IRt intermediate reticular nucleus -- ir immunoreactive -- ISN inferior salivatory nucleus -- LC locus coeruleus -- LDTg laterodorsal tegmental nucleus -- LDTgV laterodorsal tegmental nucleus ventral -- LL intermediate nucleus of the lateral lemniscus -- LPB lateral parabrachial nuclei -- LRt lateral reticular nucleus -- mcp middle cerebellar peduncle -- MdD medullary reticular nucleus -- Me5 mesencephalic trigeminal nucleus -- MG medial geniculate nucleus -- MiTg microcellular tegmental nucleus -- mlf medial longitudinal fasciculus -- MnR median raphe nuclei -- MPB medial parabrachial nuclei -- mRt mesencephalic reticular formation -- MVe medial vestibular nucleus -- PAG periaqueductal gray -- PBG parabigeminal nucleus -- PCRt parvicellular reticular nucleus -- PIL post intralaminar thalamic nuclei -- PL medial paralemniscal nucleus -- PnO pontine reticular nucleus oral part -- polyQ polyglutamine -- PP peripeduncular nucleus -- PPTg pedunculopontine tegmental nucleus -- Pr prepositus nucleus -- Pr5 principal sensory trigeminal nucleus -- PrEw pre-Edinger-Westphal nucleus -- Rbd rhabdoid nucleus -- RIP raphe interpositus nuclei -- RLi rostral linear nucleus of raphe -- RMg raphe magnus nucleus -- ROb raphe obscurus nucleus -- RPa raphe pallidus nucleus -- RRF retrorubral field -- RRID research Resource Identifier -- s5 sensory root of the trigeminal nerve -- scp superior cerebellar peduncle -- SNC substantia nigra compacta -- Sol nucleus of the solitary tract -- Sp5 spinal trigeminal nucleus -- sp5 spinal trigeminal tract -- SpVe spinal vestibular nucleus -- Su5 supratigeminal nucleus -- SubB subbrachial nucleus -- SubC subcoeruleus nucleus -- SuG superficial gray layer of the superior colliculus -- SSN superior salivatory nucleus -- TBST tris-buffered saline with 0.1% Tween -- UCN-1 urocortin-1 -- VCA ventral cochlear nucleus anterior part -- VCP ventral cochlear nucleus posterior octopus -- VSRt ventrolateral superficial reticular area -- xscp decussation of the superior cerebellar peduncle -- ZI zona incerta -- STB stigmoid body; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2022.07.016
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: The current study is the first to map STB/HAP1 immunoreactivity in the brainstem. This study also reveals the relationships of STB/HAP1 with ChAT in brainstem. STB/HAP1 is highly expressed in the brainstem preganglionic parasympathetic nuclei. STB/HAP1 is completely lacking in the cranial nerve motor nuclei in brainstem. Lack of HAP1 might make cranial nerve motoneurons vulnerable to neurodegeneration. Abstract: Huntingtin-associated protein 1 (HAP1) is a core component of stigmoid body (STB) and is known as a neuroprotective interactor with causal agents for various neurodegenerative diseases. Brain regions rich in STB/HAP1 immunoreactivity are usually spared from cell death, whereas brain regions with negligible STB/HAP1 immunoreactivity are the major neurodegenerative targets. Recently, we have shown that STB/HAP1 is abundantly expressed in the spinal preganglionic sympathetic/parasympathetic neurons but absent in the motoneurons of spinal cord, indicating that spinal motoneurons are more vulnerable to neurodegenerative diseases. In light of STB/HAP1 neuroprotective effects, it is also essential to clarify the distribution of STB/HAP1 in another major neurodegenerative target, the brainstem. Here, we examined the expression and detailed immunohistochemical distribution of STB/HAP1 and its relationships with choline acetyltransferase (ChAT) in the midbrain, pons, and medulla oblongata of adult mice. Abundant STB/HAP1 immunoreactive neurons were disseminated in the periaqueductal gray, Edinger-Westphal nucleus, raphe nuclei, locus coeruleus, pedunculopontine tegmental nucleus, superior/inferior salivatory nucleus, and dorsal motor nucleus of vagus. Double-label immunohistochemistry of HAP1 with ChAT (or with urocortin-1 for Edinger-Westphal nucleus centrally projecting population) confirmed that STB/HAP1 was highly present in parasympathetic preganglionic neurons but utterly absent in cranial nerve motor nuclei throughout the brainstem. These results suggest that due to deficient putative STB/HAP1-protectivity, cranial nerve motor nuclei might be more vulnerable to certain neurodegenerative stresses than STB/HAP1-expressing brainstem nuclei, including preganglionic parasympathetic nuclei. Our current results also lay a basic foundation for future studies that seek to clarify the physiological/pathological roles of STB/HAP1 in the brainstem.