TaEXPB5 functions as a gene related to pollen development in thermo-sensitive male-sterility wheat with Aegilops kotschyi cytoplasm. (October 2022)

• Record Type: Journal Article
• Authors: Geng, Xingxia; Wang, Xiaoxia; Wang, Jingchen; Yang, Xuetong; Zhang, Lingli; Song, Xiyue
• Is Part Of: Plant science. Volume 323(2022)
• Publication Date: 2022-10
• Subjects: Aegilops kotschyi -- Expansin protein -- Hybrid wheat -- Thermo-sensitive cytoplasmic male sterility; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2022.111377
• Languages: English
• ISSNs: 0168-9452

Abstract

Abstract: The thermo-sensitive cytoplasmic male-sterility line with Aegilops kotschyi cytoplasm (K-TCMS) is completely male sterile under low temperature (< 18 ℃) during Zadoks growth stages 45–52, whereas its fertility can be restored under hot temperature (≥ 20 ℃). The K-TCMS line may facilitate hybrid breeding and hybrid wheat production. Therefore, to elucidate the molecular mechanisms of its male sterility/fertility conversion, we conducted the association analysis of proteins and transcript expression to screen fertility related genes using RNA-seq, iTRAQ, and PRM-based assay. A gene encoding expansin protein in wheat, TaEXPB5, was isolated in K-TCMS line KTM3315A, which upregulated expression in the fertility anthers. Subcellular localization analysis suggested that TaEXPB5 protein localized to nucleus and cell wall. The silencing of TaEXPB5 displayed pollen abortion and the declination of fertility. Further, cytological investigation indicated that the silencing of TaEXPB5 induced the early degradation of tapetum and abnormal development of pollen wall. These results implied that TaEXPB5 may be essential for anther or pollen development and male fertility of KTM3315A. These findings provide a novel insight into molecular mechanism of fertility conversion for thermo-sensitive cytoplasmic male-sterility wheat, and contribute to the molecular breeding of hybrid wheat in the future. Highlights: ● TaEXPB5, a gene encoding expansin protein in wheat, was upregulated expression in KTM3315A under fertility condition. ● Silencing of TaEXPB5 displayed pollen abortion and fertility decline. ● Inhibition of TaEXPB5 expression led to early programmed cell death in the tapetum. ● TaEXPB5 was involved in pollen development.