Comparing the creamatocrit of human milk before and after long-term freezing. (24th August 2022)

• Record Type: Journal Article
• Authors: Nevels, Michael; Fujita, Masako; Brindle, Eleanor
• Is Part Of: Experimental results. Volume 3(2022)
• Publication Date: 2022-08-24
• Subjects: creamatocrit -- cryogenic storage -- fat content -- human milk -- lipid content; Science -- Experiments -- Periodicals; Science -- Methodology -- Periodicals; 507.24
• DOI: 10.1017/exp.2022.9
• Languages: English
• ISSNs: 2516-712X

Abstract

Abstract: Objectives: The creamatocrit is a simple technique for estimating the lipid content of milk, widely adopted for clinical and research purposes. We evaluated the effect of long-term cryogenic storage on the creamatocrit for human milk. Methods: Frozen and thawed milk specimens ( n = 18) were subjected to the creamatocrit technique. The specimens were reanalyzed after long-term cryogenic storage (10 years at <70°C). The correlation between pre- and post-storage values was tested, and their differences were analyzed using the Bland–Altman plot. Results: The pre- and post-storage values were highly correlated ( r = 0.960, p < .0001). The Bland–Altman plot revealed a positive association between their differences and means (Pitman's test r = 0.743, p < .001), suggesting the presence of nonconstant bias across the creamatocrit range. Long-term storage of human milk may introduce subtle bias to the creamatocrit in replicating pre-storage values. Further research should evaluate whether this bias is statistically correctable.