Complete mitochondrial genome annotation of the giant intestinal fluke, Fasciolopsis buski (Indian isolate) as revealed by ion torrent and illuminanext-generation sequencing. Issue 1 (1st January 2016)

• Record Type: Journal Article
• Authors: Biswal, Devendra Kumar; Konhar, Ruchishree; Debnath, Manish; Tandon, Veena
• Is Part Of: Mitochondrial DNA. Volume 1:Issue 1(2016)
• Page Start: 693
• Page End: 695
• Publication Date: 2016-01-01
• Subjects: Intestinal fluke -- ion torrent -- illumina -- trematode; Mitochondrial DNA -- Periodicals; 572.869
• DOI: 10.1080/23802359.2016.1222249
• Languages: English
• ISSNs: 2380-2359

Abstract

Abstract: The complete mitogenome sequences of the intestinal fluke Fasciolopsis buski are presented for the first time in this study. It is 14, 119 bp long and is thus the shortest trematode mitochondrial genome sequenced to date. The F. buski mtDNA genome has a close resemblance with F. hepatica and has a similar gene order tallying with that of other trematodes. The overall base composition of F. buski mitogenome is 17.89% for A, 9.16% for C, 27.59% for G and 45.36% for T, and has a GC content of 36.75%. The assembled mitogenome (GenBank accession number KX449331) consists of 12 protein-coding genes (PCGs), 22 transfer RNAs and two ribosomal RNA genes. The mtDNA for the intestinal fluke reported herein would help investigate Fasciolidae taxonomy and systematics with the aid of mtDNA NGS data.