Green synthesis as a simple and rapid route to protein modified magnetic nanoparticles for use in the development of a fluorometric molecularly imprinted polymer-based assay for detection of myoglobin. (10th December 2020)

• Record Type: Journal Article
• Authors: Sullivan, Mark V; Stockburn, William J; Hawes, Philippa C; Mercer, Tim; Reddy, Subrayal M
• Is Part Of: Nanotechnology. Volume 32:Number 9(2021)
• Publication Date: 2020-12-10
• Subjects: molecularly imprinted polymers -- fluorescent MIP -- fluorescent assay -- microwave -- green chemistry -- magnetic nanomaterials; Nanotechnology -- Periodicals; Nanotechnology -- Periodicals; Nanotechnology; Publications périodiques; Nanotechnologies; Periodicals; 620.5
• DOI: 10.1088/1361-6528/abce2d
• Languages: English
• ISSNs: 0957-4484

Abstract

Abstract: We have developed a low-cost molecularly imprinted polymer (MIP)-based fluorometric assay to directly quantify myoglobin in a biological sample. The assay uses a previously unreported method for the development of microwave-assisted rapid synthesis of aldehyde functionalized magnetic nanoparticles, in just 20 min. The aldehyde functionalized nanoparticles have an average size of 7.5 nm ± 1.8 and saturation magnetizations of 31.8 emu g −1 with near-closed magnetization loops, confirming their superparamagnetic properties. We have subsequently shown that protein tethering was possible to the aldehyde particles, with 0.25 ± 0.013 mg of myoglobin adsorbed to 20 mg of the nanomaterial. Myoglobin-specific fluorescently tagged MIP (F-MIP) particles were synthesized and used within the assay to capture myoglobin from a test sample. Excess F-MIP was removed from the sample using protein functionalized magnetic nanoparticles (Mb-SPION), with the remaining sample analyzed using fluorescence spectroscopy. The obtained calibration plot of myoglobin showed a linear correlation ranging from 60 pg ml −1 to 6 mg ml −1 with the limit of detection of 60 pg ml −1 . This method was successfully used to detect myoglobin in spiked fetal calf serum, with a recovery rate of more than 93%.