Β-arrestin interacts with TRAF6 to negatively regulate the NF-κB pathway in triangle sail mussel Hyriopsis cumingii. Issue 127 (August 2022)

• Record Type: Journal Article
• Authors: Jian, ShaoQing; Leng, JiangHe; Wen, ZiYi; Luo, HaiYang; Hu, ChengXi; Wen, ChunGen; Hu, BaoQing
• Is Part Of: Fish & shellfish immunology. Issue 127(2022)
• Page Start: 65
• Page End: 73
• Publication Date: 2022-08
• Subjects: Hyriopsis cumingii -- β-arrestin -- TRAF6 -- Regulation -- NF-κB pathway; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2022.06.012
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: As members of arrestins family, β-arrestins are widely expressed in monocytes, macrophages, neutrophils and other immune cells. They can regulate the immune response of bodies through various ways. In the present study, a β-arrestin homolog named Hcβ-arrestin was cloned and identified from Hyriopsis cumingii . Predicted Hcβ-arrestin protein contained a conserved arrestin domain, which could be further divided into arrestin-N (39-192aa) and arrestin-C (211-365aa). Amino acid sequence alignment showed that it had the highest identity with Mytilus galloprovincialis and Mytilus edulis counterpart, which was 89.02% and 87.68%, respectively. Furthermore, real-time quantitative PCR analysis showed that the Hcβ-arrestin gene was widely expressed in the detected tissues and with the highest expression in hepatopancreas. The transcription of Hcβ-arrestin in hepatopancreas and gill of mussels was significantly up-regulated after stimulation with peptidoglycan, lipopolysaccharide (LPS) and polyinosinic polycytidylic acid. Knockdown of Hcβ-arrestin gene significantly increased the expression of some antibacterial effector genes, such as lysozyme, LPS-binding protein/bactericidal permeability increasing protein and theromacin in hepatopancreas and gills of LPS stimulated mussels, but only had little effect on TLR pathway genes. In addition, GST pull-down assay confirmed that Hcβ-arrestin can bind to HcTRAF6 protein in vitro. Dual luciferase reporter assay showed that the co-expression of HcTRAF6 and Hcβ-arrestin inhibited the activation of NF-κB reporter by HcTRAF6. These findings indicated that Hcβ-arrestins could interact with HcTRAF6 to negatively regulate the NF-κB pathway in H. cumingii . Highlights: Hcβ-arrestin was identified from Hyriopsis cumingii . The transcription level of Hcβ-arrestin was significantly up-regulated after stimulation of PAMPs. Knockdown of Hcβ-arrestin significantly increased the expression of antibacterial genes. Hcβ-arrestin can bind to HcTRAF6 protein in vitro. Co-expression of Hcβ-arrestin and HcTRAF6 inhibited the activation of NF-κB reporter by HcTRAF6.