Long noncoding MAGI2‐AS3 promotes colorectal cancer progression through regulating miR‐3163/TMEM106B axis. Issue 5 (10th November 2019)

• Record Type: Journal Article
• Authors: Ren, Hui; Li, Zhi; Tang, Zhengjun; Li, Jun; Lang, Xiaoou
• Is Part Of: Journal of cellular physiology. Volume 235:Issue 5(2020:May)
• Page Start: 4824
• Page End: 4833
• Publication Date: 2019-11-10
• Subjects: CRC -- MAGI2‐AS3 -- miR‐3163 -- TMEM106B; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.29360
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Colorectal cancer (CRC), is mostly derived from normal colon epithelial cells, and has been reported to be one of most common gastrointestinal malignancies globally. An increasing number of researchers have claimed that long noncoding RNAs (lncRNAs) exert significant functions in tumor progression. Nevertheless, the function of MAGI2‐AS3 remains uncertain in CRC. The expression of MAGI2‐AS3, miR‐3163, and transmembrane protein 106B (TMEM106B) messenger RNA was examined by quantitative real‐time polymerase chain reaction. Cell apoptosis was measured by caspase‐3 activity test. Cell proliferation was tested by cell‐counting kit 8 and 5‐ethynyl‐2′‐deoxyuridine assays. Cell migration was detected by transwell assay. Western blot analysis examined the protein expression of TMEM106B. The expression of Ki‐67 was evaluated by immunohistochemistry assay. The binding capacity between miR‐3163 and MAGI2‐AS3 (or TMEM106B) was studied by radioimmunoprecipitation and luciferase reporter assays. The expression of MAGI2‐AS3 and TMEM106B was conspicuously upregulated whereas miR‐3163 presented lower expression in CRC cells. MAGI2‐AS3 deficiency facilitated cell apoptosis but hampered cell proliferation and migration. MAGI2‐AS3 combined with miR‐3163 and negatively regulated miR‐3163 expression. In addition, the administration of sh‐MAGI2‐AS3 or miR‐3163 mimics suppressed CRC cell growth in vivo. Subsequently, miR‐3163 targeted TMEM106B and the transfection of sh‐MAGI2‐AS3 or miR‐3163 mimics downregulated TMEM106B expression. Rescue assays verified that TMEM106B overexpression recovered the effects of MAGI2‐AS3 inhibition on cell apoptosis, proliferation, and migration in CRC. MAGI2‐AS3 drives CRC progression through regulating miR‐3163/TMEM106B axis. This supplies innovative insights on the investigation of molecular mechanism in CRC progression. Abstract : The expression of MAGI2‐AS3 and transmembrane protein 106B (TMEM106B) was conspicuously upregulated, whereas miR‐3163 presented lower expression in CRC cells. MAGI2‐AS3 deficiency facilitated cell apoptosis but hampered cell proliferation and migration. MAGI2‐AS3 combined with miR‐3163 and negatively regulated miR‐3163 expression. Either miR‐3163 amplification or MAGI2‐AS3 inhibition suppressed cell proliferative capacity in vivo.