MiR-34a and miR-125b Expression in HPV Infection and Cervical Cancer Development. (9th June 2015)

• Record Type: Journal Article
• Authors: Ribeiro, Joana; Marinho-Dias, Joana; Monteiro, Paula; Loureiro, Joana; Baldaque, Inês; Medeiros, Rui; Sousa, Hugo
• Other Names: Origoni Massimo Academic Editor.
• Is Part Of: BioMed research international. Volume 2015(2015)
• Publication Date: 2015-06-09
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2015/304584
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : We aimed to characterize miR-125b and miR-34a expression in 114 women with different cervical lesions: normal epithelium with (n = 20) and without (n = 29) HPV infection; LSIL (n = 28); HSIL (n = 29); and ICC (n = 8). miRNA expression analysis was performed by comparing the distinct groups with the reference group (women with normal epithelium without HPV). For miR-125b, we observed a twofold (2 - Δ Δ Ct = 2.11; P = 0.038) increased expression among women with normal epithelium with HPV infection and a trend of downregulation in different cervical lesions including an 80% reduction (2 - Δ Δ Ct = 0.21; P = 0.004) in ICC. Similarly, miR-34a expression analysis revealed an increased expression (2 - Δ Δ Ct = 1.69; P = 0.049) among women with normal cervix and HPV infection, and despite no significant correlation with cervical lesions, its expression was increased by twofold (2 - Δ Δ Ct = 2.08; P = 0.042) in ICC. Moreover, miR-125b levels were able to predict invasive cancers with 88% sensitivity and 69% specificity. Results showed that while miR-34a expression seems to be correlated with invasive cervical cancer, miR-125b expression is significantly changed within the different cervical lesions and their levels should be further investigated as possible predictive/prognostic biomarkers using a noninvasive approach.