A Novel Method for the Synthesis of Tc99m-Ofloxacin Kits Using D-Penicillamine as Coligand and Their Application as Infection Imaging Agent. (19th May 2015)

• Record Type: Journal Article
• Authors: Abdul Qadir, Muhammad; Shahzad, Shabnam; Rasheed, Rashid; Ahmed, Mahmood; Anwar, Shahzad; Kiran Shahzadi, Syeda
• Other Names: Kawakami Koji Academic Editor.
• Is Part Of: BioMed research international. Volume 2015(2015)
• Publication Date: 2015-05-19
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2015/502680
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : The employment of radiopharmaceuticals is increasing nowadays for infection imaging and early execution of patients having infectious or inflammatory complaints. The main aim of this study was to discover a novel method for the labeling of ofloxacin with Tc 99m, optimization of labelling conditions to get higher percent yield, to assess kits radiochemical purity, in vitro stability, partition coefficient, protein binding, and intracellular accumulation in Pseudomonas aeruginosa, Salmonella typhi, and Escherichia coli in infected rabbits. Maximum labeling efficiency was achieved when 1.5 mg ofloxacin was labeled with 10–20 mCi sodium pertechnetate in the presence of 3 mg D-penicillamine, 75 μ g SnCl2 . In vitro binding and biodistribution in Pseudomonas aeruginosa, Salmonella typhi, and Escherichia coli showed good results. This new complex is efficient for the imaging of infections caused by Gram-positive and Gram-negative bacteria.