Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution. Issue 3 (2010)

• Record Type: Journal Article
• Authors: Praus, Petr; Kocišová, Eva; Mojzeš, Peter; Štepánek, Josef; Sureau, Franck; Turpin, Pierre-Yves
• Is Part Of: Spectroscopy. Volume 24:Issue 3/4(2010)
• Page Start: 303
• Page End: 307
• Publication Date: 2010
• DOI: 10.3233/SPE-2010-0436
• Languages: English
• ISSNs: 0712-4813

Abstract

Abstract : Time-resolved confocal microspectrofluorometry and fluorescence microimaging were used to monitor how the model antisense oligonucleotide is transported into 3T3 living cells and distributed inside them. Phosphorothioate analog of 15-mer oligothymidylate labeled by ATTO 425 was complexed with Zn(II) 5, 10, 15, 20-tetrakis(4-N-methylpyridyl) porphyrin as an uptake-mediating agent. Homodyne phase-resolved technique based on a high frequency analog modulation of both exciting diode laser and detector image intensifier was used for time-resolved measurements. Decay-time data obtained within a broad range spectral region have provided unique information about the fate of both fluorophores inside the cell.