ANANASTRA: annotation and enrichment analysis of allele-specific transcription factor binding at SNPs. Issue Volume 50:Issue W1(2022) (21st April 2022)

• Record Type: Journal Article
• Authors: Boytsov, Alexandr; Abramov, Sergey; Aiusheeva, Ariuna Z; Kasianova, Alexandra M; Baulin, Eugene; Kuznetsov, Ivan A; Aulchenko, Yurii S; Kolmykov, Semyon; Yevshin, Ivan; Kolpakov, Fedor; Vorontsov, Ilya E; Makeev, Vsevolod J; Kulakovskiy, Ivan V
• Is Part Of: Nucleic acids research. Volume 50:Issue W1(2022)
• Page Start: W51
• Page End: W56
• Publication Date: 2022-04-21
• Subjects: Nucleic acids -- Periodicals; Molecular biology -- Periodicals; 572.805
• DOI: 10.1093/nar/gkac262
• Languages: English
• ISSNs: 0305-1048

Abstract

Abstract: We present ANANASTRA, https://ananastra.autosome.org, a web server for the identification and annotation of regulatory single-nucleotide polymorphisms (SNPs) with allele-specific binding events. ANANASTRA accepts a list of dbSNP IDs or a VCF file and reports allele-specific binding (ASB) sites of particular transcription factors or in specific cell types, highlighting those with ASBs significantly enriched at SNPs in the query list. ANANASTRA is built on top of a systematic analysis of allelic imbalance in ChIP-Seq experiments and performs the ASB enrichment test against background sets of SNPs found in the same source experiments as ASB sites but not displaying significant allelic imbalance. We illustrate ANANASTRA usage with selected case studies and expect that ANANASTRA will help to conduct the follow-up of GWAS in terms of establishing functional hypotheses and designing experimental verification. Graphical Abstract: