Integration of gene expression and DNA methylation identifies epigenetically controlled modules related to PM2.5 exposure. (January 2021)

• Record Type: Journal Article
• Authors: Merid, Simon Kebede; Bustamante, Mariona; Standl, Marie; Sunyer, Jordi; Heinrich, Joachim; Lemonnier, Nathanaël; Aguilar, Daniel; Antó, Josep Maria; Bousquet, Jean; Santa-Marina, Loreto; Lertxundi, Aitana; Bergström, Anna; Kull, Inger; Wheelock, Åsa M.; Koppelman, Gerard H.; Melén, Erik; Gruzieva, Olena
• Is Part Of: Environment international. Volume 146(2021)
• Publication Date: 2021-01
• Subjects: Air pollution -- DNA methylation -- Gene expression -- Integration -- Children; Environmental protection -- Periodicals; Environmental health -- Periodicals; Environmental monitoring -- Periodicals; Environmental Monitoring -- Periodicals; Environnement -- Protection -- Périodiques; Hygiène du milieu -- Périodiques; Environnement -- Surveillance -- Périodiques; Environmental health; Environmental monitoring; Environmental protection; Periodicals; 333.705
• DOI: 10.1016/j.envint.2020.106248
• Languages: English
• ISSNs: 0160-4120

Abstract

Highlights: This genome-wide study investigated the association of gene expression with PM2.5 . Two differentially expressed transcripts were associated with PM2.5 exposure at birth. Integration of gene expression and DNA methylation identified hubs linked to PM2.5 . Our study shows the added value of integrating environmental and multi-omics data. Abstract: Air pollution has been associated with adverse health effects across the life-course. Although underlying mechanisms are unclear, several studies suggested pollutant-induced changes in transcriptomic profiles. In this meta-analysis of transcriptome-wide association studies of 656 children and adolescents from three European cohorts participating in the MeDALL Consortium, we found two differentially expressed transcript clusters (FDR p < 0.05) associated with exposure to particulate matter < 2.5 µm in diameter (PM2.5 ) at birth, one of them mapping to the MIR1296 gene. Further, by integrating gene expression with DNA methylation using Functional Epigenetic Modules algorithms, we identified 9 and 6 modules in relation to PM2.5 exposure at birth and at current address, respectively (including NR1I2, MAPK6, TAF8 and SCARA3). In conclusion, PM2.5 exposure at birth was linked to differential gene expression in children and adolescents. Importantly, we identified several significant interactome hotspots of gene modules of relevance for complex diseases in relation to PM2.5 exposure.